钴(Ⅲ)的联吡啶类混配配合物的合成,表征及其与DNA键合的光谱研究

合成了两种新的钴多吡啶类混配配合物。通过元素分析 ,红外 ,紫外 ,核磁方法对其进行表征 ,应用吸收及发射光谱 ,DNA熔点技术 ,循环伏安法 ,研究了其与小牛胸腺DNA的作用。结果显示 ,混配配合物与DNA作用时 ,使紫外吸收明显减色 ,荧光显著增强 ,DNA熔点升高。循环伏安法研究表明 ,配合物仅有还原峰 ,与DNA作用后 ,还原峰电流明显下降。这些结果证明配合物与DNA存在插入结合 ,Fe(CN) 6 ]4 - 猝灭实验也支持这一结论。峰电位有位移 ,说明配合物与DNA分子中带负电荷的磷酸基团可能存在静电结合 ,进而计算得到配合物与DNA的键合常数分别为 1 8× 10 4 ,4 37× 10 4 。

Synthesis Characterization and DNA Binding Spectroscopy Studies of Cobalt(Ⅲ) Mixed-polypyridyl Complex

Two complexes ofCo(phen)2PIP](ClO4)3.H2O and Co(phen)2HPIP](ClO4)3.2H2O have been synthesized(PIP = 2-imidazo4,5-f]1,10]phenanthroline, HPIP = 2-(2-hydroxyphenyl)-imidazo 4,5-f]1,10]phenanthroline) and characterized by elemental analysis, UV, IR and 1H NMR spectroscopy. The interaction of the complexes with calf thymus DNA have been studied using absorption and emission spectroscopy, DNA melting techniques and cyclic voltammetric. The compounds show absorption hypochromicity, fluorescence enhancement, DNA melting increasement when binding to CT.DNA. CV measurement shows that the shift in reduction potential and the change in peak current with addition of DNA. These results prove the compounds insert DNA base pairs. The shift of peak potential indicates the ion interaction mode between the drug and DNA. The binding constants of the compounds to DNA are 1.8 x 10(4) and 4.37 x 10(4) respectively.