| 激光引发DNA及多聚核苷酸与SO4.-作用的闪光光谱研究 |
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| 引用本文： | 宋钦华,李全新,徐业平,戴静华,俞书勤,陈从香,马兴孝,林维真,姚思德.激光引发DNA及多聚核苷酸与SO4.-作用的闪光光谱研究[J].光谱学与光谱分析,1999,19(4):521-524. |
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| 作者姓名： | 宋钦华 李全新 徐业平 戴静华 俞书勤 陈从香 马兴孝 林维真 姚思德 |
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| 作者单位： | 1. 中国科学技术大学化学物理系,230026,合肥
2. 中国科学院上海原子核研究所辐射化学开放实验室,201800,上海 |
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| 基金项目： | 中国博士后科学基金;; |
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| 摘 要： | 运用２４８ｎｍ激光光解瞬态吸收光谱研究了ＳＯ４·－与ＤＮＡ及其核苷酸和多聚核苷酸ｐｏｌｙ［Ｇ］、ｐｏｌｙ［Ａ］和ｐｏｌｙ［Ｃ］的相互作用，观察了各体系的作用过程，结果表明，ｐｏｌｙ［Ｇ］、ｐｏｌｙ［Ａ］与ＳＯ４·－发生单电子氧化反应生成嘌呤端基自由基；ｄＣＭＰ与ＳＯ４·－单电子氧化生成阳离子自由基，随后与水反应生成５－羟基－６Ｃ自由基；而ｐｏｌｙ［Ｃ］，ＳＯ４·－先在碱基上加成，生成的加成自由基再从核糖上抽氢生成Ｃ２’－糖自由基；ＤＮＡ与ＳＯ４·－作用生成的主要瞬态产物是鸟嘌呤端基自由基。
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| 关 键 词： | 激光闪光光谱 SO4.-单电子氧化 自由基 |
Study on Laser Flash Spectroscopy of Laser-induced Interaction of DNA and Polynucleotides with SO4.- |
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| Qinhua SONG,Quanxin LI,Yeping XU,Jinghua DAI,Shuqin YU,CHEN Congxiang,Xingxiao MA,Weizhen LIN,Side YAO.Study on Laser Flash Spectroscopy of Laser-induced Interaction of DNA and Polynucleotides with SO4.-[J].Spectroscopy and Spectral Analysis,1999,19(4):521-524. |
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| Authors: | Qinhua SONG Quanxin LI Yeping XU Jinghua DAI Shuqin YU CHEN Congxiang Xingxiao MA Weizhen LIN Side YAO |
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| Institution: | Department of Chemical Physics, University of Science and Technology of China, 230026 Hefei. |
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| Abstract: | The interactions of DNA and polynucleotides (polyG],polyA] and polyC]) with SO4*- were investigated by 248 nm laser flash photolysis. The results show that, the reactions of polyG] or polyA] with SO4*- yield predominantly the corresponding one-electron-oxidated base radicals. With polyC], it is suggested that SO4*- radical reacts predominantly by addition to the base moiety to produce C5-yl and C6-yl sulphate radical adducts,and subsequently H-abstraction yields C2'-sugar radical. The predominant species resulting from the reaction of DNA with SO4*- is DNA guanyl radical. |
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| Keywords: | DNA |
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