| 脉冲电场与牛血清白蛋白相互作用的同步荧光光谱和拉曼光谱比较研究 |
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| 引用本文： | 李乐军,陈树德,乔登江.脉冲电场与牛血清白蛋白相互作用的同步荧光光谱和拉曼光谱比较研究[J].光谱学与光谱分析,2006,26(1):81-85. |
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| 作者姓名： | 李乐军 陈树德 乔登江 |
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| 作者单位： | 华东师范大学光谱学与波谱学教育部重点实验室,华东师范大学物理系,上海,200062 |
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| 基金项目： | 中国科学院资助项目
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上海市科技发展基金 |
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| 摘 要： | 应用同步荧光光谱和拉曼光谱研究了脉冲电场与牛血清白蛋白的相互作用.同步荧光光谱研究表明,脉冲电场对牛血清白蛋白的发射荧光光谱强度产生影响,降低了处于其活性部位的色氨酸和酪氨酸残基的发射荧光强度.拉曼光谱进一步验证了这种实验结果.两种实验表明:脉冲电场改变了处于牛血清白蛋白活性中心的芳香族氨基酸所处的微环境,进而表明了蛋白质的构象发生了变化,从而影响它的生物学功能.
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| 关 键 词： | 同步荧光光谱 拉曼光谱 脉冲电场(PEF) 牛血清白蛋白(BSA) |
| 文章编号： | 1000-0593（2006）01-0081-05 |
| 收稿时间： | 2004-08-15 |
| 修稿时间： | 2005-01-20 |
Synchronous Fluorescence and Raman Spectroscopy Study on the Interaction of Pulsed Electric Field (PEF) and Bovine Serum Albumin (BSA) |
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| LI Le-jun,CHEN Shu-de,QIAO Deng-jiang.Synchronous Fluorescence and Raman Spectroscopy Study on the Interaction of Pulsed Electric Field (PEF) and Bovine Serum Albumin (BSA)[J].Spectroscopy and Spectral Analysis,2006,26(1):81-85. |
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| Authors: | LI Le-jun CHEN Shu-de QIAO Deng-jiang |
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| Institution: | Key Laboratory of Optical and Magnetic Resonance Spectroscopy, Department of Physics, East China Normal University, Shanghai 200062, China |
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| Abstract: | The interaction of pulsed electric field (PEF) and bovine serum albumin (BSA) was studied by synchronous fluorescence and Raman spectroscopy. The results of synchronous fluorescence showed that pulsed electric field exerted its effects on the emission fluorescence spectrum and reduced the fluorescence intensities of the tyrosine and tryptophan side chains. The results of Raman spectroscopy verified this. These two experiments indicated that PEF exposure changed the microenvironments of the two aromatic amino acids, which were located in the active parts of BSA, and further indicated the conformational changes of the proteins, and the change in its biological functions. |
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| Keywords: | Synchronous fluorescence spectroscopy Raman spectroscopy Pulsed electric field Bovine serum albumin (BSA) |
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