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血卟啉与人血清白蛋白结合作用特征的热力学研究
引用本文:张勇,燕新梁,雷亚春,沈晓芳,潘景浩,刘滇生.血卟啉与人血清白蛋白结合作用特征的热力学研究[J].光谱学与光谱分析,2005,25(8):1274-1276.
作者姓名:张勇  燕新梁  雷亚春  沈晓芳  潘景浩  刘滇生
作者单位:山西大学化学化工学院,山西,太原,030006
基金项目:国家自然科学基金(20375024)和山西省青年科技基金(20021008)资助项目
摘    要:采用荧光光谱法和紫外-可见吸收光谱法研究了在模拟人体生理条件下不同温度时,血卟啉与人血清白蛋白结合反应的荧光猝灭光谱行为,根据Stern-Volmer方程和Lineweaver-Burk双倒数函数曲线计算了其结合常数在高温时要比低温时小;根据Fφster能量传递原理计算出结合位点r=4.36,并根据热力学参数确定了它们之间的作用力类型主要为氢键和VanderWaals力,HP进入了HSA的疏水腔。

关 键 词:人血清白蛋白  血卟啉  荧光光谱法  吸收光谱法  热力学参数
文章编号:1000-0593(2005)08-1274-03
收稿时间:12 18 2004 12:00AM
修稿时间:05 16 2005 12:00AM

Thermodynamics Studies on the Reaction Characteristics of Hemoporphyrin with Human Serum Albumin
ZHANG Yong,YAN Xin-liang,LEI Ya-chun,SHEN Xiao-fang,PAN Jing-hao,LIU Dian-sheng.Thermodynamics Studies on the Reaction Characteristics of Hemoporphyrin with Human Serum Albumin[J].Spectroscopy and Spectral Analysis,2005,25(8):1274-1276.
Authors:ZHANG Yong  YAN Xin-liang  LEI Ya-chun  SHEN Xiao-fang  PAN Jing-hao  LIU Dian-sheng
Institution:College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Abstract:The quenching reaction of hemoporphyrin with human serum albumin (HSA) was studied byusing fluorescence spectraand absorption spectra. The formation constants of them were analyzed at different temperature according to Stem-Volmer equation and double-reciprocal equation, which are smaller at high temperature than at low temperature. The binding site was calculated (r = 4.36) by Foster energy transfer mechanism, and the thermodynamic parameters were obtained. H-bond and Van der Waals are two main reactions between HP and HSA.
Keywords:Human serum albumin  Hemoporphyrin  Fluorescence spectra  Absorption spectra  Thermodynamic parameters
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