近红外光谱法测定不同产地黄芪中毛蕊异黄酮葡萄糖苷和黄芪甲苷含量

采用近红外漫反射光谱法对黄芪中毛蕊异黄酮葡萄糖苷和黄芪甲苷的含量进行快速无损检测。以液相色谱质谱联用分析值为参比，采用偏最小二乘法建立黄芪中毛蕊异黄酮葡萄糖苷和黄芪甲苷的定量分析模型。结果显示，毛蕊异黄酮葡萄糖苷近红外光谱经多元散射校正(MSC)+一阶导数+Savitzky-Golay卷积平滑预处理后模型最优，模型参数R2为0.826 6，RMSEP值为0.022 7，校正集R2为0.863 5，RMSEC值为0.019 0；黄芪甲苷近红外光谱经二阶导数+Savitzky-Golay卷积平滑预处理后模型最优，模型参数R2为0.854 8，RMSEP值为0.006 41，校正集R2为0.796 3，RMSEC值为0.007 99。近红外光谱技术结合偏最小二乘法可快速、准确的对黄芪中毛蕊异黄酮葡萄糖苷和黄芪甲苷的含量进行检测。此外，通过主成分分析，发现甘肃产黄芪与其他产地黄芪差异不大，排除甘肃产黄芪后，山西、四川和吉林的样本区分度较高。

Determination of Calycosin-7-Glucoside and Astragaloside in Astragali Radix with Near Infrared Spectroscopy

A quick and nondestructive method using near-infrared(NIR) diffuse reflectance spectroscopy was presented for the determination of the contents of calycosin-7-glucoside and astragaloside in astragali radix.With liquid chromatograph/mass spectrometer analysis of the reference value,partial least squares method was applied to establish a quantitative analysis model on calycosin-7-glucoside and astragaloside.The results showed that the model of calycosin-7-glucoside had optimal performance with pretreatment method of multiplicative scatter correction,first derivative and Savitzky-Golay convolution smoothing.The determination coefficients was 0.826 6 whiel the root mean square prediction(RMSEP)was 0.022 7;determination coefficients of calibration set was 0.863 5 and the root mean square error of calibration (RMSEC) was 0.019 0.Astragaloside after second derivative and Savitzky-Golay convolution smoothing,the determination coefficients was 0.854 8,the root mean square prediction was 0.006 41;determination coefficients of calibration set was 0.796 3 and the root mean square error of calibration was 0.007 99.Studies have shown that near infrared spectroscopy combined with partial least squares method is rapid,reliable and it could be implemented in the content detection of Calycosin-7-glucoside and astragaloside in Astragali Radix.In addition,In addition,through the analysis of Principal Component analysis,it can be found little difference between the regions of Gansu and non-Gansu,there will be higher degree of differentiation among Shanxi,Sichuan and Jilin after elimination the region of Gansu Astragalus.