健康人体乳腺傅里叶变换红外光谱特征参数分析

通过应用傅里叶变换红外光谱仪(FTIR)的中红外光导纤维并联合衰减全反射探头(ATR)对招募的200名女性健康志愿者的右侧乳腺外上限部位皮肤进行扫描采集相应的傅里叶变换红外光谱,由此共获取200条正常人体乳腺的傅里叶变换红外光谱图。进一步对所得的200条正常人体乳腺傅里叶变换红外光谱图中与脱氧核糖核酸、核糖核酸、蛋白质、脂类、糖类等生物化学成分相关的12条特征谱带进行分析研究,并对这些特征谱带的峰位(P)、峰强(I)、半高宽(F)等36个特征性红外光谱参数进行统计学分析,同时计算相应特征红外光谱参数的90%正常参考值范围、均值、标准差等数值。研究结果首次对正常人体乳腺傅里叶变换红外光谱图相关特征光谱参数的数据建立了正常参考范围,同时为傅里叶变换红外光谱技术进一步实现其在乳腺良恶性疾病诊断方面无创、快速、高效的特有临床应用价值提供相应的理论依据。

Analysis of Characteristic Parameters of the Surface Fourier Transform Infrared Spectra from Normal Human Breast

A total of two hundred cases of surface Fourier transform infrared (FTIR) spectra from normal female breast were obtained by scanning two hundred enrolled healthy volunteers' upper limit of the right breast outside the skin with two mid-infrared optical fibers and an attenuated total reflectance (ATR) probe .Twelve characteristic bands of the above FTIR spectra,related to deoxyribonucleic acid,ribonucleic acid,proteins,lipids and carbohydrate were analyzed .Thirty-six spectral parameters of these characteristic bands,including peak positions (P),intensities (I) and full width at half maximum (F) of FTIR spectrums,were statistically analyzed .Meantime,90％ nor mal reference value range,mean value,standard deviation of above characteristic parameters was obtained and calculated .In present study,we had establish the normal reference range of spectral parameters of characteristic bands in the surface Fourier transform infrared spectra scanned from normal female breast,for the first time .Meanwhile,for further implementation of Fourier transform infra red spectroscopy technique in noninvasive,fast and efficient characteristic clinical diagnosis of breast benign and malignant diseases,these research results was of great importance .Eventually,this study also had made an attempt to explore the profound theoretical basis in the biomedical application of Fourier transform infrared spectroscopy technique .