| 溴鼠灵与DNA作用机制的光谱研究 |
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| 引用本文： | 段云青,闵顺耕.溴鼠灵与DNA作用机制的光谱研究[J].光谱学与光谱分析,2009,29(4):999-1003. |
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| 作者姓名： | 段云青 闵顺耕 |
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| 作者单位： | 1. 中国农业大学理学院,北京,100094;山西农业大学文理学院,山西,太谷,030801
2. 中国农业大学理学院,北京,100094 |
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| 基金项目： | 国家自然科学基金，山西高校科技研究开发基金 |
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| 摘 要： | 农用化学物质残留可能会与DNA结合形成加和物,从而对动物和人类造成危害.文章利用紫外光谱和荧光光谱研究了溴鼠灵(BDF)与小牛胸腺DNA(ct-DNA)的作用机理.结果表明,低浓度的ct-DNA可使BDF的吸收光谱发生减色,ct-DNA对BDF的稳态荧光有明显的的猝灭作用,属于静态猝灭方式,27℃时Ksv=1.21×104L·mol-1;根据热力学参数推测BDF与ct-DNA之间的相互作用主要是范德瓦耳斯力作用;猝灭试验发现ct-DNA对BDF无明显保护作用;离子强度改变时,BDF/ct-DNA体系的荧光强度也随之改变;ct-DNA对β-CD/BDF包络物的荧光也有猝灭,但猝灭程度比游离的BDF有所降低,表明β-CD对BDF具有保护作用,也说明BDF与ct-DNA或β-CD的结合具有竞争性.各试验结果一致表明BDF与ct-DNA之间主要是以沟区结合方式发生作用.另外,试验结果也表明BDF与ct-DNA之间还存在静电作用.范德瓦耳斯力和静电作用的共同作用,为BDF在沟区与DNA的相互结合提供了更好条件.
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| 关 键 词： | 溴鼠灵 小牛胸腺DNA 荧光光谱 紫外光谱 沟区结合 |
| 收稿时间： | 2007/11/16 |
Study of the Interaction Mechanism between Brodifacoum and DNA by Spectroscopy |
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| DUAN Yun-qing,MIN Shun-geng.Study of the Interaction Mechanism between Brodifacoum and DNA by Spectroscopy[J].Spectroscopy and Spectral Analysis,2009,29(4):999-1003. |
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| Authors: | DUAN Yun-qing MIN Shun-geng |
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| Institution: | DUAN Yun-qing1,2,MIN Shun-geng1 1. College of Science,China Agricultural University,Beijing 100094,China 2. College of Art , Science,Shanxi Agricultural University,Taigu 030801,China |
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| Abstract: | The interaction between brodifacoum (3-3-(4'-bromophenyl-4)1,2,3,4-tetralin-10]-4-hydroxyl-coumarin) (BDF),an anticoagulant rodenticide,and calf thymus DNA (ct-DNA) was studied by UV spectrum and fluorescence spectrum. The results were summarized as follows:There was a hypochromic effect of low concentration ct-DNA on the UV spectra. The fluorescence quenching studies showed a regular decrease in the fluorescence intensity after addition of ct-DNA by the static quenching mode with a quenching constant (KSV... |
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| Keywords: | Brodifacoum Calf thymus DNA Fluorescence spectrum UV spectrum Groove binding |
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