火焰原子吸收光谱法测定中草药丹参不同部位金属元素的含量

采用HNO3-HClO4(4:1)混酸消化丹参的根、茎和叶,利用火焰原子吸收光谱法对消化液中的五种人体必需的金属元素K,Cu,Zn,Fe,Mg进行了测定.该方法的标准曲线的相关系数为0.998 7～0.999 7,加标回收率为99.7%～104.4%,相对标准偏差(RSD)小于2%.结果显示:K,Cu,Zn,Fe,Mg在丹参根、茎和叶的含量均有以下次序:K＞Mg＞Fe＞Zn＞Cu.丹参叶的K,Cu,Fe,Mg的含量大于根和茎.根、茎、叶中的锌铜比值均较小.该测定结果为研究金属元素K,Cu,Zn,Fe,Mg在丹参中的分布以及金属元素的含量与丹参药效之间的关系提供了有用的数据.

Determination of Metallic Elements in Different Parts of Salvia Miltiorrhiza by FAAS

The root, stem and leaf of Salvia miltiorrhiza were digested with HNO3-HClO4 (4:1), and the contents of five metallic elements such as K, Cu, Zn, Fe and Mg were detected by flame atomic absorption spectrophotometry. The recovery ratio by standard addition is 99.7%-104.4% and RSD is less than 2%. It was found that the content sequence of metallic elements is as follows: K>Mg >Fe>Zn>Cu. K, Cu, Zn, Fe and Mg in the leaf are clearly richer than those in the root and stem. The content ratio of Zn to Cu is relatively lower than that in the terrestrial angiosperms. The results provide a useful data for discussing the relationship between the contents of these elements in Chinese medicinal herb Salvia miltiorrhiza and the medical effects. Furthermore they also provide a data for the distribution of these elements in different parts of Salvia miltiorrhiza.