| 化学发光法测定尿酸 |
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| 引用本文： | 刘二保,卫洪清.化学发光法测定尿酸[J].光谱学与光谱分析,2005,25(8):1213-1215. |
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| 作者姓名： | 刘二保 卫洪清 |
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| 作者单位： | 1.天津师范大学化学与生命科学学院, 天津 300074
2.山西师范大学化学与材料科学学院, 山西 临汾, 041004 |
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| 基金项目： | 国家自然科学基金（20075017）,天津师范大学学科建设基金资助 |
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| 摘 要： | 利用尿酸对碱性luminol-H2O2-Co2+化学发光体系具有较强的抑制作用，建立了一种测定尿酸的新方法。方法的线性范围为1.0×10-10～7.0×10-6 mol·L-1，检测限(3σ)为1.1×10-11 mol·L-1，RSD 1.9%(n=4，ｃs=5.0×10-8 mol·L-1)。该法用于人体血清及尿液中尿酸的测定，结果满意。
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| 关 键 词： | 化学发光 尿酸 Co2+ |
| 文章编号： | 1000-0593（2005）08-1213-03 |
| 收稿时间： | 2004-10-26 |
| 修稿时间： | 2005-01-10 |
Determination of Uric Acid by Chemiluminescence |
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| LIU Er-bao,WEI Hong-qing.Determination of Uric Acid by Chemiluminescence[J].Spectroscopy and Spectral Analysis,2005,25(8):1213-1215. |
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| Authors: | LIU Er-bao WEI Hong-qing |
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| Institution: | 1.School of Chemistry and Life Sciences, Tianjin Normal University, Tianjin 300074, China2.School of Chemistry and Material Science, Shanxi Normal University, Linfen 041004, China |
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| Abstract: | A novel chemiluminescence method for the determination of uric acid based on its inhibitory effect on the catalysis of Co2+ for the oxidation of luminol by H2O2 is described. The proposed method is simple, very sensitive and inexpensive. The decrease of cheniluminescence intensity is linearly correlated with uric acid concentration. The calibration graph is linear over the range of 1.0 x 10(-10) - 7.0 x 10(-6) mol x L(-1) and the detection limit is 1.1 x 10(-11) mol x L(-1). The relative standard deviation (RSD) for 5.0 x 10(-8) is 1.9% (n=4). The method has been successfully applied to the determination of uric acid in human urine and serum samples. |
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| Keywords: | Chemiluminescence Uric acid Co~(2 ) |
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