荧光光谱及其成像技术在光活检中的应用

分析了人体组织自体荧光的主要物质来源和荧光特性，同时总结了国内外常见的第二代新型光敏剂及其临床应用情况。在此基础上，全面比较了各种应用于早期肿瘤光活检的荧光光谱及其成像技术，并重点讨论了这些技术的基本原理和临床应用现状，以及它们今后的发展方向。

Fluorescence Spectroscopy and Imaging for Optical Biopsy

In the past few years extensive attention has turned toward optical biopsy, because of its ability to perform noninvasive, real time and accurate diagnosis of tissue pathologies, especially precancer. Fluorescence spectroscopy is one of a series of techniques widely developed for optical biopsy, and can be generally divided into two categories: fluorescence spectroscopy and imaging. This paper (reviews) the applications of autofluorescence and exogenous fluorescence to optical biopsy, and the principle and advantages as well as the clinic applications of various steady-state and time-resolved fluorescence spectroscopy and imaging techniques. Furthermore, the development perspectives of optical biopsy by using fluorescence spectroscopy and imaging are also briefly discussed.