| 同步荧光、导数荧光与卡尔曼滤波荧光光度法用于混合荧光染料同时测定的比较研究 |
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| 引用本文： | 张晶玉,杨金夫,任英,张岩,符连社,雷中利.同步荧光、导数荧光与卡尔曼滤波荧光光度法用于混合荧光染料同时测定的比较研究[J].光谱学与光谱分析,1994(4). |
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| 作者姓名： | 张晶玉 杨金夫 任英 张岩 符连社 雷中利 |
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| 作者单位： | 中国科学院长春应用化学研究所，长春冶金地质专科学校 |
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| 摘 要： | 本文以荧光光谱重叠的罗丹明Ｂ和罗丹明６Ｇ二组分混合体系为例考察了卡尔曼滤波法处理荧光光谱数据与荧光光谱法中的同步荧光技术和数值导数方法对该体系荧光光谱的解析能力。由于该体系中二组分谱峰重叠程度较轻，采用同步荧光技术可以将二者完全分开。用卡尔曼滤波法处理常规荧光光谱数据也获得了令人满意的结果。但该体系的一阶导数光谱不仅不能将二者分开，而且灵敏度降低，不及前二种方法。
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| 关 键 词： | 荧光分光光度，同步荧光法，导数荧光法，卡尔曼滤波法 |
A COMPARATIVE STUDY OF KALMAN FILTERING, SYN CHRONOUS EXCITATION AND NUMERICAL DERIVATIVE TECHNIQUES IN THE SIMULTANEOUS DETERMINATION OF MIXTURE OF FLUORESCENT DYE IN FLUOROSPECTROPHOTOMETRY |
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| ZHANG Jingyu,YANG Jinfu,REN Ying,ZHANG Yan,FU Lianshe and LEI Zhongli.A COMPARATIVE STUDY OF KALMAN FILTERING, SYN CHRONOUS EXCITATION AND NUMERICAL DERIVATIVE TECHNIQUES IN THE SIMULTANEOUS DETERMINATION OF MIXTURE OF FLUORESCENT DYE IN FLUOROSPECTROPHOTOMETRY[J].Spectroscopy and Spectral Analysis,1994(4). |
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| Authors: | ZHANG Jingyu YANG Jinfu REN Ying ZHANG Yan FU Lianshe and LEI Zhongli |
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| Abstract: | The performance of kalman filtering,synchronous excitation and numerical derivative techniques for the resolution of overlapping emission spectra in fluorospectrophotometry was experimentally studied.Because of the small overlapping extent of the spectra of rhodamin B and rhodamin 6G,both Kalman filtering and synchronous excitation techniques were able to resolve the overlapping spectra well and gave satisfactory results,while the derivative spectra were still overlapped with each other.Moreover,the sensitivities of the dervative spectra were greatly decreased. |
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| Keywords: | Fluorospectrophotometry Synchronous fluorescence Kalman filtering Numerical derivative Fluorescent dye |
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