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胞嘧啶与3,3',5,5'-四溴间甲酚磺酞反应体系研究
引用本文:迟燕华,白进伟,庄稼.胞嘧啶与3,3'''',5,5''''-四溴间甲酚磺酞反应体系研究[J].光谱学与光谱分析,2005,25(11):1839-1842.
作者姓名:迟燕华  白进伟  庄稼
作者单位:西南科技大学材料科学与工程学院化学与生物工程系,四川,绵阳,621010
基金项目:教育部科学技术研究重点项目(02126);国家电分析化学重点实验室基金项目(2005007)和四川省教育厅自然科学基金项目(2002C04)资助
摘    要:采用UV-Vis法对胞嘧啶(Cy)与3,3',5,5'-四溴间甲酚磺酞(XJFL)反应体系进行了研究.研究了在酸性条件下温度、时间、离子强度等对反应体系的影响;测得反应的线性范围为0~32μg·mL-1,最大结合数n=16,摩尔吸光系数ε=1.32×103 L·cm-1·mol-1等;并对反应机理进行了研究.同时测定了无机物,生物物质,表面活性剂对反应体系的干扰情况.

关 键 词:胞嘧啶  3  3’  5  5’-四溴间甲酚磺酞  UV—Vis法  Tris-HCl缓冲溶液
文章编号:1000-0593(2005)11-1839-04
收稿时间:08 8 2004 12:00AM
修稿时间:11 28 2004 12:00AM

Study on the Chemical Reaction of Cytosine with 3,3', 5,5' -Tetrabromo-m-cresojsulphonphthalein
CHI Yan-hua,BAI Jin-wei,ZHUANG Jia.Study on the Chemical Reaction of Cytosine with 3,3'''', 5,5'''' -Tetrabromo-m-cresojsulphonphthalein[J].Spectroscopy and Spectral Analysis,2005,25(11):1839-1842.
Authors:CHI Yan-hua  BAI Jin-wei  ZHUANG Jia
Institution:College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China.
Abstract:The system of reaction for t he cytosine (Cy) and 3,3',5,5'-tetrabrono-m-cresojsulphonphthalein (XJFL) was studied in acid solution using UV spectra. The factors that affect the reaction, such as temperature, time and concentration of ion were determined. The maximum absorption peak of the complex appeared at t he wavelength 620 nm, the molar absorptivity was epsilon = 1.32 x 10(3) L x cm(-1) x mol(-1), the linear range of standard was 0-32 micro x mL(-1), and the maximum combining number was 16. The reaction mechanism, and the interaction of inorganic substance, biological substance, and surface active agent were studied.
Keywords:Cytosine  3  3'  5  5'-tetrabromo-m-cresojsulphonphthalein  Spectrophotometry  Tris-HCl buffer solution
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