荧光偏振光谱法探测光动力过程中癌细胞膜的流动性

以DPH作荧光探剂，用荧光偏振光谱法研究了金属酞菁配合物苯硫基钛菁锌(C₅₆H₃₂N₈S₄Zn)在光动力过程中，不同光照时间对乳腺癌细胞膜流动性的影响。实验结果表明，经光照激发光敏剂金属酞菁后，荧光标记团的偏振度增大，癌细胞膜流动性降低，微黏度升高。说明这是光动力治疗的疗效所在。用酶联免疫检测(MTT法)不同光照时间对细胞存活的影响，结果显示癌细胞最低存活率与膜流动性降低成正相关。这表明光动力过程在一个相近的程度影响癌细胞膜的流动性和增殖，提示光动力过程可能通过影响细胞膜流动性引起细胞增殖变化，即细胞膜是光动力作用的一个位点。

Fluorescence Polarization Used to Derive Cell Membrane Fluidity during Photodynamic Therapy

With metal phthalocyanine used as the photosensitizer, the cell membrane fluidity under the conditions of different irradiation time in photedynamic therapy was investigated by fluorescence polarization. Results show that with the photosensitizer phthalocyanine excited, the fluorescence polarization of the fluorophose was increased and the cancer cell membrane fluidity was decreased due to the photodynamic therapy. Cell proliferation was also studied by MTT. Results show that cell membrane fluidity and cell proliferation are influenced to the same extent, suggesting that cell membrane is one of the binding sites in PDT.