| 双嘧达莫与牛血清白蛋白结合热力学特征的荧光光谱法研究 |
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| 引用本文： | 颜承农,上官云凤,童金强,刘义,庞代文,潘祖亭,屈松生.双嘧达莫与牛血清白蛋白结合热力学特征的荧光光谱法研究[J].光谱学与光谱分析,2003,23(3):543-546. |
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| 作者姓名： | 颜承农 上官云凤 童金强 刘义 庞代文 潘祖亭 屈松生 |
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| 作者单位： | 1. 荆州师范学院化学系,湖北,荆州,434020
2. 武汉大学化学与分子科学学院,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金 (2 99730 30 )，湖北省教育厅重点项目 (2 0 0 1A41 0 0 5)资助 |
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| 摘 要： | 用荧光光谱法和紫外 可见吸收光谱法研究了在不同温度下 ,双嘧达莫与牛血清白蛋白结合反应的荧光猝灭光谱行为 ,得出了结合常数、结合位点和基本热力学参数。
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| 关 键 词： | BSA 双嘧达莫 荧光猝灭光谱 热力学参数 |
| 文章编号： | 1000-0593(2003)03-0543-04 |
| 修稿时间： | 2002年3月6日 |
Study on Thermodynamics of Binding Reaction of Dipyridamole with Bovine Serum Albumin |
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| YAN Cheng-nong,SHANGGUAN Yun-feng,TONG Jin-qiang,LIU Yi,PANG Dai-wen,PAN Zu-ting and QU Song-sheng.Study on Thermodynamics of Binding Reaction of Dipyridamole with Bovine Serum Albumin[J].Spectroscopy and Spectral Analysis,2003,23(3):543-546. |
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| Authors: | YAN Cheng-nong SHANGGUAN Yun-feng TONG Jin-qiang LIU Yi PANG Dai-wen PAN Zu-ting and QU Song-sheng |
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| Institution: | Department of Chemistry, Jinzhou Normal University, Jinzhou 434020, China. |
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| Abstract: | The binding feature of dipyridamole with bovine serum albumin (BSA) was studied using fluorescence spectroscopy. It was shown that this compound has a powerful ability to quench the BSA fluorescence via a nonradiative energy transfer mechanism. The fluorescence quenching data were analyzed according to stern-volmer equation and double-reciprocal equation,and the binding constant and the thermodynamic parameters were obtained. |
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| Keywords: | Bovine serum albumin Dipyridamole Fluorescence spectrum Thermodynamic parameters |
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