微波消解-原子吸收法分析并比较四种贝母中的微量元素

利用火焰原子吸收分光光度法(FAAS)及石墨炉原子吸收分光光度法(GFAAS)测定了浙贝、象贝、东贝、川贝4种贝母中15种微量元素的含量.同时考察了不同微波消解条件对分析结果的影响,确定了原子吸收分光光度法测定的最佳条件,并且以微量元素含量作为指标对其进行了主成分分析.实验结果表明,微波消解法操作简便、快速、样品消解完全,空白值低,选择性好,方法相对标准偏差小于3.96%,回收率在91.0%～108.7%.主成分分析法在反映不同样品微量元素含量的差异程度上具有应用价值.

Determination and Comparison of Trace Elements in Four Sorts of Bulbus Fritillariae by Microwave Digestion-Atomic Absorption Spectrometry

The contents of elements such as Zn,Fe,Mn,Cu,Na,Mg,K,Ca,As,Pb,Cd,Co,Cr,Sr,and Al in four sorts of Bulbus Fritillariae,namaly from Fritillaria thunbergii Miq.(Panan and Ningbo),Fritillaria thunbergii Miq.Var.chekiangensis Hsiao et K.C.Hsia and Fritillaria cirrhosa D.Don,were determined by flame atomic absorption spectrometry(FAAS) and graphite furnace atomic absorption spectrometry(GFAAS) respectively.The effect of different microwave digestion conditions on the analysis results was reviewed,and optimal condition for using atomic absorption spectrophotometry was determined.Principal component analysis(PCA) was applied taking trace elements contents as indexes.The results revealed that microwave digestion was a simple,rapid,digestion complete,and low blank value method,and the measurement result is satisfactory.The experimental results indicated that the linear relationships for different elements within the limits of working curve were good.The RSDs were all smaller than 3.97%.The addition standard rates of the procedure was between 91.0% and 108.7%.Principal component analysis has the application value of reflecting the differences in trace elements contents in various samples.