| 抗-转铁蛋白-纳米金探针的制备及对转铁蛋白免疫识别的光谱分析 |
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| 引用本文： | 杨培慧,韦炜,蔡怀鸿,冯竟,蔡继业.抗-转铁蛋白-纳米金探针的制备及对转铁蛋白免疫识别的光谱分析[J].光谱学与光谱分析,2009,29(5):1398-1401. |
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| 作者姓名： | 杨培慧 韦炜 蔡怀鸿 冯竟 蔡继业 |
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| 作者单位： | 暨南大学化学系,广东,广州,510632 |
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| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
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| 摘 要： | 利用碳二亚胺(EDC)将抗-转铁蛋白化学偶联到已修饰了半胱胺的纳米金表面,制备了抗-转铁蛋白-纳米金免疫探针,应用共振瑞利散射光谱,紫外-可见吸收光谱,透射电镜和激光散射等方法对其进行了表征.所制备的纳米探针具有良好的免疫活性.由于抗-转铁蛋白对转铁蛋白抗原具有特异性识别能力,借助免疫纳米探针在470 nm处共振瑞利散射信号的放大作用,对转铁蛋白抗原进行特异性识别及免疫分析.转铁蛋白浓度在0.85～33.9×10-10mol·L1范围内,470 nm处共振瑞利散射相对强度与转铁蛋白浓度呈良好的线性关系,检测下限为8.5×10-11moL·L-1.
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| 关 键 词： | 抗-转铁蛋白-纳米金探针 共振瑞利散射光谱 免疫识别 |
| 收稿时间： | 2008/2/2 |
Immobilization of Anti-Transferrin On Nano-Gold and Its Immune Recognition of Transferrin |
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| YANG Pei-hui,WEI Wei,CAI Huai-hong,FENG Jing,CAI Ji-ye.Immobilization of Anti-Transferrin On Nano-Gold and Its Immune Recognition of Transferrin[J].Spectroscopy and Spectral Analysis,2009,29(5):1398-1401. |
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| Authors: | YANG Pei-hui WEI Wei CAI Huai-hong FENG Jing CAI Ji-ye |
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| Institution: | YANG Pei-hui,WEI Wei,CAI Huai-hong,FENG Jing,CAI Ji-ye Department of Chemistry,Jinan University,Guangzhou 510632,China |
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| Abstract: | A novel strategy based on antigen-antibody interaction was developed by means of backfilling transferrin on antibody functionalized gold nanoparticles surfaces in the present study.Nano gold particles were immobilized with cysteamine layer by self-assembly,whose surfaces were chemically coupled with anti-transferrin antibodies by using EDC,forming the anti-transferrin-Au immuno-probes.The particles and the nano-probes were characterized by the integrated tools of resonance Rayleigh scattering(RRS),UV-Vis ab... |
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| Keywords: | Anti-transferrin-Au probe Resonance Rayleigh scattering Immune recognition |
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