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玫苯胺共振光散射法测定DNA
引用本文:刘晨,陈小明,向海艳,李松青,夏殊,刘安喜.玫苯胺共振光散射法测定DNA[J].光谱学与光谱分析,2001,21(5):697-700.
作者姓名:刘晨  陈小明  向海艳  李松青  夏殊  刘安喜
作者单位:湘潭大学化学化工学院,
基金项目:湖南省自然科学基金资助项目 (资助号 0 0JJY2 0 84)
摘    要:本文研究了三苯甲烷类碱性染料玫苯胺与DNA作用的共振光散射光谱 ,在 pH =10 5~ 11的范围内 ,DNA的加入导致玫苯胺共振光散射的增强 ,在 4 85nm处 ,存在一共振光散射增强峰 ,其强度与DNA的浓度呈线性关系 ,据此建立了一种测定DNA的共振光散射法。方法的线性范围为 0~ 1 0 0mg·L-1,检测限为14 2ng·mL-1。将该方法用于混合样品中DNA的测定 ,结果令人满意。

关 键 词:玫苯胺  共振光谱散射  DNA  测定  光谱分析
文章编号:1000-0593(2001)05-0697-04
修稿时间:2000年2月29日

Determination of Deoxyribonucleic Acid with Rosaniline by a Resonance Light Scattering Method
C Liu,X M Chen,H Y Xiang,S Li,S Xia,A X Liu.Determination of Deoxyribonucleic Acid with Rosaniline by a Resonance Light Scattering Method[J].Spectroscopy and Spectral Analysis,2001,21(5):697-700.
Authors:C Liu  X M Chen  H Y Xiang  S Li  S Xia  A X Liu
Institution:Institute of Chemistry and Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
Abstract:The resonance light scattering (RLS) spectra of Rosaniline with DNA have been studied. The RLS of Rosaniline is greatly enhanced by DNA in pH range of 10.5-10.8. There is a resonance light scattering peak ofat 485 nm, and the enhanced intensity of RLS at this wavelength is proportional to the concentration of DNA. The linear range of the calibration graph is 0-1.00 mg.L-1 with the correlation coefficient of 0.9980. The detection limit is 14.2 ng.mL-1. The relative standard deviation(n = 10) was within 2.1%. This method is simple, rapid and has been applied to the determination of DNA in mixed samples with satisfactory results.
Keywords:Rosaniline  Resonance light scattering method  DNA  
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