利用傅里叶变换红外光谱法鉴定小麦品种

利用傅里叶变换红外(FTIR)光谱法分析了8个不同小麦品种根部的红外光谱图,对红外光谱图的吸收峰进行了归属分析,通过对各谱图的比对发现,在相同波数范围内,不同的小麦品种其红外谱图的形状、波数及吸收峰的多少有所不同。结果表明不同小麦品种的红外光谱图有明显的差异,其一在2800～2980cm-1波数范围内,此区反映的主要是甲基、亚甲基的伸缩振动,化合物分子链的长短、分子量的大小在此区有显现;其二差别较大的区域在1510～1730cm-1范围内,它反映的主要是酰胺的氮原子及α碳原子取代基的性质,可能是源于基因的差异,此区域内谱图差异较大。因此FTIR光谱法可用于不同小麦品种的鉴别。

Identification of Wheat Varieties by FTIR Spectrum

The spectra of eight kinds of wheat roots were determined by FTIR spectrometry and the absorption peaks were analyzed. Results from the comparison of the spectra showed that there were some differences in the shape, wave number and the number of absorption peaks of their spectra within the same range of wave number. The spectra of different wheat varieties exhibited remarkable differences. The first difference was in the region of 2800-2980 cm(-1) which mainly reflected the stretching vibration of methyl and methylene, the length of molecular chain of compound, and the molecular weight. The second difference existed in the region of 1510-1730 cm(-1), which mainly reflected the substituent nature of amide N and alpha-C. The major differences of spectra in this region might result from the differences in genes. So FTIR spectra could be used for the identification of wheat varieties.