| 傅里叶变换显微红外光谱法研究羟自由基与红细胞膜脂和膜蛋 … |
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| 引用本文： | 周群,张声俊.傅里叶变换显微红外光谱法研究羟自由基与红细胞膜脂和膜蛋 …[J].光谱学与光谱分析,1998,18(2):162-166. |
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| 作者姓名： | 周群 张声俊 |
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| 摘 要： | 利用傅里叶变换显微红外光谱法和计算机辅助解析法研究了羟自由基作用于红细胞后膜蛋白二级结构的变化规律及羟自由基对红细胞膜脂质损伤的作用机理。结果表明，由于羟自由基的攻击，导致表征蛋白质二级结构的α－螺旋的含量发生变化；自由基损伤３０分钟后，放置３小时其蛋白结构仍不能恢复；
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| 关 键 词： | 红细胞膜脂 羟自由基 膜蛋白 损伤 自由基 膜脂 |
FTIR microscope study on the change of membrane protein and lipids of erythrocyte damaged by free radicals] |
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| Q Zhou,S Sun,S Zhang,X Hu,Y Huang,H Zhao,D Liu.FTIR microscope study on the change of membrane protein and lipids of erythrocyte damaged by free radicals][J].Spectroscopy and Spectral Analysis,1998,18(2):162-166. |
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| Authors: | Q Zhou S Sun S Zhang X Hu Y Huang H Zhao D Liu |
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| Institution: | Analysis Center, Tsinghua University, 100084 Beijing. |
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| Abstract: | The FTIR Microscope and Computer Aided Analysis were used to study not only the change of secondary structure of erythrocyte membrane protein attacked by the free radicals, but also the effect of oxygen centered free radicals on the membrane lipids in this paper. The results indicate that the content of alpha-helix in the secondary structure of protein clearly changed because of the attack of the free radicals. Damaged by free radicals in 30 minutes, the protein structure could not be restored after 3 hours. The number of the P=O (vp=0), C=O and C=C in the lipids of the membrane was changed. These changes were caused by the peroxidation of the oxygen centered free radicals. |
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