人体血清血糖浓度对血清荧光强度的影响

血液在临床医学诊断和治疗中有着重要的价值，很多疾病都可能导致血液成分或性质发生特征性的变化。因此，对血液中的糖进行分析将是十分重要且有实用意义的。文章研究了正常血清与高血糖血清在光激发下荧光强度的差异。结果表明在365 nm处荧光图像的平均灰度值随血糖的浓度增大呈现明显增大的趋势；但在353，405, 369 nm处的荧光图像的平均灰度变化不大。这表明葡萄糖主要影响血清365 nm处的荧光强度。由于荧光强度与荧光物质的浓度有关，所以血糖浓度变化直接影响365 nm处的荧光强度。因此，可以根据365 nm处的荧光强度的大小来判断血清中的血糖含量是否超标，这为光学技术应用于血液疾病诊断方面提供实验依据。

Effect of Glucose Concentration in Human Serum on Fluorescence Intensity

Blood plays an important role in the clinical diagnosis and treatment; many diseases may lead to changes in the blood composition or characteristic properties. Therefore, the analysis of blood sugar will be of great importance. The present paper studies the difference of fluorescence intensity stimulated by light wave between normal blood serum and hyperglycemia blood serum. The result shows that the average grey level of fluorescence image at 365 nm increases obviously with the changes in the concentration of glucose; however, the average grey changes little at 353, 369 and 405 nrn. This shows that the main effect of serum glucose on the fluorescence intensity peak appears at 365 nm. Because the fluorescence intensity has something to do with the concentration of the fluorescence material, the blood glucose concentration influences directly the fluorescence intensity at 365 nm. Therefore, it is possible to judge blood sugar content, and to make sure whether the content exceeds the normal value or not by comparing the fluorescence intensity of the blood serum measured at 365 nm. This technique of optical measurement provides an experimental basis for the diagnosis of blood diseases.