航天育种甜椒、番茄品系种子的FTIR分析

采用傅里叶变换红外光谱法(FTIR)，分析研究了航天育种甜椒、番茄品系种子与大田生产常用的甜椒品种、系统法选育的番茄品系种子的特征和变异。初步探索了空间效应对甜椒、番茄种子变异的影响。红外吸收光谱表明，四个航天种子产生的共同变异是: 1 160～1 061 cm-1范围峰的吸收都比对照品系增大，表明空间诱变效应使C—O振动增强。特殊变异是：(1)太空甜椒的2 854 cm-1峰(ν_s)增强，表明CH₂的碳氢对称伸缩振动增强;(2)太空小番茄的1 162 cm-1(ν_s)增强显著，可能由碳水化合物的C—O的伸缩振动峰增强引起。可以初步认为，太空甜椒、番茄种子的共同变异受太空微重力的因素影响较大，而其特殊变异则可能主要与太空强辐射的因素相关。

Novel Application of FTIR to the Analysis of Sweet Pepper Seeds and Tomato Seeds with Space Mutation

Comparative study of the sweet pepper and tomato seeds lines with space mutation and corresponding conventional seeds was carried out with FTIR, in an attempt to search for the influence of space environment upon mutation. The similarities among space seeds lines are: the absorbance at near 1,160-1,061 cm(-1) increased. This result shows that the stretching vibration of C-O on the structure of carbohydrates was enhanced by space mutation. The special mutations are: (1) The absorbance at 2,854 cm(-1) of space sweet pepper was enhanced, showing that the stretching vibration of CH2 was stronger; (2) The absorbance at 1,160 cm(-1) of cherry tomato seeds lines was increased notably, indicating that the stretching vibration of C-O on the structure of carbohydrates was stronger. The results show that the space environment could notably increase the carbohydrates in the tomato seeds. The space sweet pepper and tomato seeds lines, which produced similar mutation, could be influenced by microgravity in space. The special mutations are the results of the influence of universe radiation in space.