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光谱法研究3H-吲哚季铵盐探针分子与牛血清底的相互作用
引用本文:徐同宽,沈兴海,高宏成.光谱法研究3H-吲哚季铵盐探针分子与牛血清底的相互作用[J].光谱学与光谱分析,2005,25(6):857-860.
作者姓名:徐同宽  沈兴海  高宏成
作者单位:北京大学化学与分子工程学院,北京,100871
基金项目:国家自然科学基金(90206020, 29901001)资助项目
摘    要:合成了一种新型的荧光探针分子2-(对-十二烷基氨基)苯基-3, 3-二甲基-5-乙酯基-3H-吲哚基-甲基-二-十六烷基碘化铵, 并运用荧光光谱探讨它与牛血清蛋白的作用. 结果表明, 探针分子与牛血清蛋白(BSA)的结合作用可使3H-吲哚的荧光强度增强, 而对蛋白质却具有猝灭性质, 其结合常数和结合位点数分别为Ka=1.995×105 dm3*mol-1和n=1.12.

关 键 词:取代3H-吲哚季铵盐  合成  牛血清蛋白  荧光光谱
文章编号:1000-0593(2005)06-0857-04
修稿时间:2003年10月28

Synthesis of Substituted 3H-Indole Quaternary Ammonium Salt and Study on Its Interaction with Bovine Serum Albumin by Fluorescence
XU Tong-kuan,SHEN Xing-hai,GAO Hong-cheng.Synthesis of Substituted 3H-Indole Quaternary Ammonium Salt and Study on Its Interaction with Bovine Serum Albumin by Fluorescence[J].Spectroscopy and Spectral Analysis,2005,25(6):857-860.
Authors:XU Tong-kuan  SHEN Xing-hai  GAO Hong-cheng
Institution:College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Abstract:A substituted 3H-indole quaternary ammonium molecule was designed and synthesized using hexamethylphosphoramide (HMPA) as a solvent. The products were purified and characterized by IR, 1H-NMR, MS and elemental analysis. The binding reaction of this compound with bovine serum albumin (BSA) in aqueous solution was studied using fluorescence. Their binding constant is Ka = 1.995 x 10(5) dm3 x mol(-1) and the binding site number is n = 1.12. It is confirmed that the combination is a single static quenching process.
Keywords:Substituted 3H-indole quaternary ammonium molec ule  Synthesis  BSA  Fluorescence
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