| 副品红共振光散射法测定脱氧核糖核酸 |
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| 引用本文： | 向海艳,陈小明,周迪武.副品红共振光散射法测定脱氧核糖核酸[J].光谱学与光谱分析,2002,22(6):1051-1053. |
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| 作者姓名： | 向海艳 陈小明 周迪武 |
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| 作者单位： | 1. 株洲电力机车研究所印制电路板厂,湖南,株洲,412001
2. 湘潭大学化学化工学院,湖南,湘潭,411105 |
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| 摘 要： | 本文基于脱氧核糖核酸 (DNA)对有机染料副品红的共振光散射的增强效应 ,拟定了一种测定DNA的共振光散射法。在pH =0 5～ 1 5范围内 ,有机染料副品红于 35 5nm处的共振光散射强度被DNA强烈增强 ,且增强程度与DNA浓度呈线性关系 ,线性范围为 0 10～ 15 μg·mL- 1 ,检出限可达 36ng·mL- 1 。该方法简便、快速、具有较高的灵敏度和准确度 ,且线性范围较宽。将该方法用于混合样品中DNA的测定 ,结果令人满意。
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| 关 键 词： | 测定 副品红 脱氧核糖核酸 共振光散射法 DNA |
| 文章编号： | 1000-0593(2002)06-1051-03 |
| 修稿时间： | 2001年3月12日 |
Determination of Deoxyribonucleic Acid with Pararosaniline by a Resonance Light-scattering Method |
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| Hai-yan Xiang,Xiao-ming Chen,Di-wu Zhou.Determination of Deoxyribonucleic Acid with Pararosaniline by a Resonance Light-scattering Method[J].Spectroscopy and Spectral Analysis,2002,22(6):1051-1053. |
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| Authors: | Hai-yan Xiang Xiao-ming Chen Di-wu Zhou |
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| Institution: | Printed Circuit Board Factory, Zhuzhou Electric Locomotive Institute, Zhuzhou 412001, China. |
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| Abstract: | The method is based on the enhancement effect of the resonance light-scattering (RLS) of pararosaniline by DNA. In the pH rang of 0.5-1.5, the enhanced intensity of pararosaniline RLS at 355 nm is proportional to the concentration of DNA in the rang of 0.20-15 micrograms.mL-1. The detection limit is 36 ng.mL-1. This method is simple, rapid and has been applied satisfactorily to the determination of DNA in mixed samples. |
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| Keywords: | Pararosaniline Deoxybribonucleic acid Resonance light scattering method |
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