炮附片、黑顺片和白附片二维红外相关光谱的分析研究

采用红外光谱法(FTIR)并结合二维相关(Two-dimensional Correlation Spectroscopy)分析技术,对炮附片、黑顺片和白附片进行了无损快速鉴别研究。炮附片、黑顺片和白附片在一维谱图上差别不明显,而在二维谱图上凭借各官能团相互作用强度的不同而可以进行一一鉴别。在900～1500cm~(-1)波段内,炮附片在同步图上有一个较强的自动峰,在1220cm~(-1)附近。而黑顺片和白附片不仅在1220cm~(-1)有一个较强的自动峰,还在1200和1400cm~(-1)附近出现了自动峰,且白附片在1400cm~(-1)附近的自动峰比黑顺片的要更强一些;在交叉线的两侧,三者各官能团正的交叉峰白附片最多,黑顺片次之,炮附片最少。结果表明:凭借炮附片、黑顺片和白附片的二维红外相关谱可以作为药材不同炮制法的鉴别依据。该法快速、准确,为中药材的炮制鉴别提供了一种新的方法和手段。

Study on Paofupian, Heishunpian and Baifupian by Two-dimensional Infrared Correlation Spectroscopy

Paofupian, Heishunpian and Baifupian, three kinds of Radix Aconiti Lateralis Preparata with different processing procedures, were identified fast and nondestructively by Fourier Transform Infrared spectroscopy (FTIR) combined with Two-Dimensional Infrared Correlation Spectroscopy (2D FTIR) in this paper. The three kinds of samples were similar to each other in one-dimensional IR spectra but quite different in 2D FTIR spectra. In the range between 900 and 1 500 cm-1, one strong autopeak was aroused by Paofupian around 1 220 cm-1 in synchronous spectrum, whereas three autopeaks not only at 1 220 cm but also at 1 200 cm-1 and 1 400 cm-1 were aroused by Heishunpian and Baifupian. Also the autopeak at 1 400 cm-1 in Baifupian was stronger than that of Heishunpian. It can be seen clearly that the crosspeaks in these synchronous spectra were quite different too. It is proved that the 2D FTIR could serve in distinguishing Paofupian, Heishunpian and Baifupian. Therefore, the fast and accurate property of 2D FTIR makes it a powerful and new approach to identifying the different procedures to process medicinal herbs.