色氨酸和酪氨酸的三维荧光光谱特征参量提取

氨基酸是维持生命活动的重要物质，而色氨酸和酪氨酸又是天然氨基酸中重要的发光组分，应用荧光光谱法对其进行测量和分辨具有重要的意义。文章用美国Pekin-Elmer LS55型荧光分光光度计，对色氨酸和酪氨酸的三维荧光光谱进行了测量。将测量的数据用激发-发射-荧光强度的三维坐标表示，得到三维荧光谱图，但色氨酸和酪氨酸存在共性峰，通过波峰位置简单地来辨别两种混叠的物质很有难度。以数理统计概念为基础，提取该三维荧光光谱的特征参数，得到两种物质荧光光谱中最相关的信息，可以解决两种物质光谱混叠的分辨问题。结果表明，色氨酸和酪氨酸的三维荧光光谱平均值、标准差、原点矩、混合中心矩等参数差值百分比分别为330.37%, 102.86%, 329.16%, 329.63%，区别较大;而边际分布、相关系数值差值百分比仅为10.61%和2.40%。因而平均值、标准差、原点矩、混合中心矩可作为敏感特征参数，用其分辨谱图混叠的色氨酸和酪氨酸是可行的。这种“数学预提取”的三维光谱分析法可以找出组分之间的敏感特征参量，能够取代传统的三维荧光光谱分析法。

Extraction of Characteristic Parameters of Three-Dimensional Fluorescence Spectra of Tyrosine and Tryptophan

In the present paper,three-dimensional fluorescence spectra of tyrosine and tryptophan were detected through Pekin-Elmer LS55 fluorescence spectrometer made in U.S.A,with the excitation wavelength in the range of 230-320 nm,interval of 5 nm,emission wavelength in the range of 230-500 nm,and interval of 2 nm.Three-dimensional fluorogram was obtained using excitation wavelength,emission wavelength and fluorescence intensity detected by Pekin-Elmer LS55 as three-dimensional system of coordinate.It can be seen ...