| 温度对单个活态人红细胞携氧能力的即时影响 |
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| 引用本文： | 姚成灿,李校坤,黄耀熊.温度对单个活态人红细胞携氧能力的即时影响[J].光谱学与光谱分析,2005,25(4):613-616. |
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| 作者姓名： | 姚成灿 李校坤 黄耀熊 |
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| 作者单位： | 1. 暨南大学药学院，广东 广州 510630
2. 暨南大学生物医学工程研究所，广东 广州 510630 |
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| 基金项目： | 国家自然科学基金仪器专项 (30 2 2 70 0 1 )，面上基金 (60 3770 4 3)资助项目 |
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| 摘 要： | 利用快速显微多道分光光度技术，在无损、在位、实时的情况下，测定了不同环境温度下单个活态人红细胞内血红蛋白吸收光谱的即时变化情况。发现人红细胞内血红蛋白吸收峰的高度和位置均随环境温度的变化而变化，表明人红细胞的携氧能力与温度密切相关。初步揭示了人红细胞内血红蛋白分子结构、浓度与功能之间的密切关系，并且探讨了温度变化对人红细胞生理功能即时影响的分子机制。
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| 关 键 词： | 快速显微多道分光光度技术 红细胞 携氧能力 温度 |
| 文章编号： | 1000-0593(2005)04-0613-04 |
| 收稿时间： | 2004-09-16 |
| 修稿时间： | 2004年9月16日 |
Instant Effect of Temperature on the Oxygen Carrying Capacity of Single Living Intact Red Blood Cell |
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| YAO Cheng-can,LI Xiao-kun,HUANG Yao-xiong.Instant Effect of Temperature on the Oxygen Carrying Capacity of Single Living Intact Red Blood Cell[J].Spectroscopy and Spectral Analysis,2005,25(4):613-616. |
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| Authors: | YAO Cheng-can LI Xiao-kun HUANG Yao-xiong |
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| Institution: | 1. College of Pharmacy, Jinan University, Guangzhou 510630, China2. Institute of Bioengineering, Jinan University, Guangzhou 510630, China |
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| Abstract: | The instant effect of temperature on the absorption spectra of the hemoglobin in single living intact red blood cells was investigated , by employing a highly sensitive fast multi-channel micro-spectrophotometer system to perform non-invasive, in situ , real time measurements on the cells. It was found that both the heights and position of the specific peaks in the absorption spectra of intercellular hemoglobin were changed with temperature, indicating that the oxygen carrying capacity of red blood cells varies with temperature. The correlations of the structure and concentration as well as the function of hemoglobin, and the molecular mechanism were also discussed. |
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| Keywords: | Fast multi-channel micro-spectrophotometry Red blood cell Oxygen carrying capacity Temperature |
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