盐酸丁卡因与赤藓红的荧光猝灭反应及其分析应用

在pH4.0的弱酸性介质中,盐酸丁卡因(TA.HCl)与赤藓红(ET)形成1:1的离子缔合物,导致赤藓红溶液荧光猝灭。当分别于最大激发/最大发射(λex/λem)525nm/556nm进行测量时,荧光猝灭值(ΔF)与TA.HCl浓度在0.28～4.8μg.mL-1范围呈良好的线性关系。该方法灵敏度高,检出限为0.083μg.mL-1。考察了共存物质的影响,表明方法有良好的选择性。据此发展了一种高灵敏、简便快速测定微量TA.HCl的新荧光分光光度法。该法用于人血清及尿样中盐酸丁卡因的含量测定,结果满意。文章还研究了反应体系的荧光特性,并结合量子化学AM1计算对荧光猝灭机理进行了讨论。

Fluorescence Quenching Reaction of Tetracaine Hydrochloride with Erythrosine and Their Analytical Applications

In Britton-Robinson (BR) buffer solution at pH 4.0, erythrosine (ET) reacts with tetracaine hydrochloride (TA x HCl) to form an ion association complex that quenches the fluorescence of erythrosine and has a compound ratio of 1 : 1. The measurement wavelength of fluorescence quenching was located at lambda(ex)/lambda(em) = 525 nm/556 nm. The linear range for TA x HCl is from 0.28 microg x mL(-1) to 4.8 microg x mL(-1), and its detection limit is 0.083 microg x mL(-1). The influence of coexisting substance was also inspected, and the result shows that this method has a better selectivity. Therefore, a new fluorospectrophotometry, being high sensitive, sample and rapid, was developed to determinate TA x HCl at trace amounts. The method has been applied to the determination of TA x HCl in human serum and urine samples with satisfactory results. In this study, the spectral characteristics and the optimum reaction conditions were discussed with quantum chemistry AM1 method.