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| 肺正常组织与癌变组织的表面增强拉曼光谱 | |
| 引用本文： | 刘燕楠,邹祖全,刘燕青,徐晓轩,俞钢,张存洲.肺正常组织与癌变组织的表面增强拉曼光谱[J].光谱学与光谱分析,2007,27(10):2045-2048. |
| 作者姓名： | 刘燕楠 邹祖全 刘燕青 徐晓轩 俞钢 张存洲 |
| 作者单位： | 1.南开大学物理科学学院光子学中心，天津 300071 2.南开大学物理科学学院生物物理系，天津 300071 3.南开大学弱光非线性光子学材料先进技术及制备教育部重点实验室，天津 300457 4.武警医学院病理学教研室， 天津 300162 |
| 摘 要： | 采用共焦显微拉曼光谱进行肺正常组织与癌变组织的表面增强拉曼光谱测试，拉曼位移测试范围为300～1700cm-1 。根据得到的肺正常和癌变组织的表面增强拉曼光谱，对比分析了两者的特点与差异。选取蛋白质主链构象中构象不灵敏的CH2弯曲振动在1454cm-1的谱线为内标，发现相比肺正常组织，癌变组织蛋白质主链中酰胺Ⅲ和酰胺Ⅰ谱带的有序构象明显减少，对α螺旋和无规则卷曲的损伤比较严重，β回折几乎消失，而骨架C—N，C—C伸缩振动却出现了与前2个谱带截然相反的状况，有序构象显著增加，蛋白质侧链构象变化与主链相比则相对复杂；DNA的骨架磷酸基团、脱氧核糖、磷酸-脱氧核糖和碱基的含量总体呈增加趋势；RNA的情况与DNA类似；磷脂的链内纵向有序性参数明显下降。 |
| 关 键 词： | 表面增强拉曼光谱 肺正常组织 肺癌变组织 |
| 文章编号： | 1000-0593（2007）10-2045-04 |
| 收稿时间： | 2006-08-02 |
| 修稿时间： | 2006-08-02 |
Surface-Enhanced Raman Spectra of Natural Tissue and Cancerous Tissue of Lung |
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| LIU Yan-nan,ZOU Zu-quan,LIU Yan-qing,XU Xiao-xuan,YU Gang,ZHANG Cun-zhou.Surface-Enhanced Raman Spectra of Natural Tissue and Cancerous Tissue of Lung[J].Spectroscopy and Spectral Analysis,2007,27(10):2045-2048. | |
| Authors: | LIU Yan-nan ZOU Zu-quan LIU Yan-qing XU Xiao-xuan YU Gang ZHANG Cun-zhou |
| Institution: | 1. Photonics Center of School of Physics, Nankai University, Tianjin 300071, China2. Department of Biophysics, School of Physics, Nankai University, Tianjin 300071, China3. The Key Laboratory of Advanced Technique and Fabrication for Weak-Light Nonlinear Photonics Materials, Ministry of Education, Nankai University, Tianjin 300457, China4. Pathology Staff Room of Medical College Chinese People’s Armed Police Force, Tianjin 300162,China |
| Abstract: | Surface-enhanced Raman spectra of natural tissue and cancerous tissue of lung from 300 to 1700 cm(-1) were measured. In the cancerous tissue of lung, the orderly conformations of amides III and amides I of the main chains in protein such as alpha-helix, beta-corner and no rules curly were damaged seriously; conversely, the extendable vibration of skeleton C-N and skeleton C-C increased. Side chains change became complicated. In general, the content of nucleic acids increases in the cancer tissue of lung. The lengthways conformations of lecithoid chains is out-of-order in the cancerous tissue of lung compared with the natural ones. |
| Keywords: | Surface-enhanced Raman spectra Natural tissue of lung Cancerous tissue of lung |
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