Au标蛋白自组装表面增强拉曼光谱的研究

采用免疫Au标技术，用尺寸大约在13 nm的Au胶体颗粒标记了人血清蛋白(Human IgG)，然后将Au标蛋白复合体固定在通过三氨基三乙氧基硅烷和戊二醛自组装单膜的Si片上。这种方法在基底表面上不仅牢固地固定了单层Au纳米颗粒标记的蛋白分子复合体而且提高了复合体的表面覆盖度，保持了生物分子的结构。利用原子力显微镜(AFM)观察了Au标蛋白的自组装表面。实验结果表明Au标蛋白在Si片表面聚积形成一定的Au标蛋白分子的复合体“岛状”单层，并且在这些岛状单层上获得了很明显的标记蛋白的表面增强拉曼散射(SERS)信号。文章在Si表面自组装了Au标蛋白分子，获得了较好的蛋白分子的SERS信号，提供了一种研究蛋白分子的SERS活性基底。

Surface Enhanced Raman Spectroscopic Study on the Gold-Labeled Protein Self-Assembled Surface

The human IgG molecules were labeled with 13 nm gold nanoparticles and the complex of the gold-labeled human IgG molecules was immobilized on a silicon surface modified by 3-aminopropyltriethoxysilane and glutaraldehyde.The method increases not only the tightness but also the surface coverage for immobilization of the complex and retains protein configuration well on the silicon surface.The self-assembled complex surface was observed by AFM.The complex aggregated on the silicon surface and the "island" monolayer of the complex was obtained.Meanwhile the SERS spectrum of the complex self-assembled "island" monolayer on silicon surface was presented.In the present paper,the gold labeled human IgG molecules were self-assembled on the silicon surface,SERS spectra of protein were obtained and as SERS active substrates were provided for the study of the protein molecules.