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| 鉴别直肠组织癌变的环丙沙星铽络合物荧光染色新方法 | |
| 引用本文： | 孟涛,徐端夫,赵莹,王笃金,徐怡庄,齐剑,吴瑾光.鉴别直肠组织癌变的环丙沙星铽络合物荧光染色新方法[J].光谱学与光谱分析,2007,27(10):2069-2072. |
| 作者姓名： | 孟涛 徐端夫 赵莹 王笃金 徐怡庄 齐剑 吴瑾光 |
| 作者单位： | 1. 中国科学院化学研究所高分子物理与化学国家重点实验室,北京,100080;北京印刷学院,北京,102600 2. 中国科学院化学研究所高分子物理与化学国家重点实验室,北京,100080 3. 北京大学化学与分子工程学院,北京,100871 |
| 基金项目： | 国家重点基础研究发展计划(973计划) , 国家自然科学基金 |
| 摘 要： | 用具有较强荧光性能的环丙沙星铽配合物对多例直肠正常和直肠癌组织切片进行了染色研究,并与传统的HE染色结果进行了对比.结果表明用环丙沙星铽配合物染色后的效果与HE染色具有相同的图像和光学反差.并同时通过测定直肠正常和直肠癌组织的荧光光谱和红外光谱得到的组织病变判据,其结论与染色结果相一致. |
| 关 键 词： | 铽络合物 环丙沙星 荧光染色 直肠癌 |
| 文章编号： | 1000-0593（2007）10-2069-04 |
| 修稿时间： | 2006-08-10 |
New Fluorescent Staining Method to Distinguish Normal and Malignant Rectum Tissues Using Terbium (Ⅲ)-Ciprofloxacin Complex |
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| MENG Tao,XU Duan-fu,ZHAO Ying,WANG Du-jin,XU Yi-zhuang,QI Jian,WU Jin-guang.New Fluorescent Staining Method to Distinguish Normal and Malignant Rectum Tissues Using Terbium (Ⅲ)-Ciprofloxacin Complex[J].Spectroscopy and Spectral Analysis,2007,27(10):2069-2072. | |
| Authors: | MENG Tao XU Duan-fu ZHAO Ying WANG Du-jin XU Yi-zhuang QI Jian WU Jin-guang |
| Institution: | 1. State Key Laboratory of Polymer Physics and Chemistry, Joint Laboratory of Polymer Science and Materials, Institute of Chemistry, the Chinese Academy of Sciences, Beijing 100080, China; 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China; 3. Beijing Institute of Graphic Communication, Beijing 102600, China |
| Abstract: | A new fluorescent staining method to distinguish normal and malignant rectum tissues using terbium(III)-Ciprofloxacin complex was investigated. The terbium (III) complex exhibits strong fluorescence and the staining process with the complex is very fast. It needs 3-5 minutes. The results showed that the fluorescence microscope images were similar to the images stained using traditional hematoxylin and eosin (H&E) methodology. The fluorescence spectra and FTIR spectra of normal and malignant rectum tissues were collected and the detection criterions for the malignant rectum tissues were the same as the staining results. In summary, the fluorescence staining may be developed as a simple and convenient method. |
| Keywords: | Terbium complexes Ciprofloxacin Fluorescence staining Rectal cancer |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |