刺五加不同部位的红外光谱分析与鉴定

采用红外光谱三级宏观指纹鉴定的方法对刺五加不同部位根、茎、叶原药材及总苷提取物的红外光谱图进行了整体的分析。原药材谱图显示，根和茎谱图较为相似，主要体现草酸钙和淀粉特征峰，而叶的草酸钙特征峰几乎消失，淀粉特征峰的峰形也变得不明显。总苷提取物的一维谱图显示根、茎、叶在1 602，1 514，1452 cm-1(苯环骨架振动)和1 271 cm-1(CO)附近均有特征吸收，因此，推测三者共有的成分为酚苷类化合物。二阶导数谱图给出叶在1 656 cm-1(黄酮的CO)附近特征峰明显强于根和茎，说明叶中黄酮类成分高于根和茎。二维谱图在1 350～1 700 cm-1波段根和茎均体现苯环骨架振动的5个自动峰1 636， 1 600，1 521，1 462，1 453 cm-1,叶除了苯环骨架振动峰外还出现1 656 cm-1黄酮类成分的CO振动，进一步证实叶中黄酮类成分高于根和茎。

Analysis of Acanthopanax Senticosus Harms for Different Parts Using Fourier Transform Infrared Spectroscopy

In the present paper,raw radix,stem and leaf and the total glycoside extract of them of Acanthopanax senticosus Harms were studied via the multi-steps IR macro-fingerprint method.The spectra of raw medicinal materials show that the spectra of radix and stem are similar and mainly show characteristic peaks of calcium oxalate and starch,whereas the characteristic peaks of calcium oxalate of leaf almost disappear and the shape of characteristic peak of starch also gets unconspicuous.The FTIR spectra of total glycoside extract of radix,stem and leaf present characteristic peaks at 1 602,1 514,1 452 cm-1(vibration of phenyl framework) and 1 271 cm-1(CO),respectively,therefore,the authors speculated that their mutual component is the compound of phenolic glycoside.Through observing the second derivative IR spectra of the total glycoside extract of different parts of Acanthopanax senticosus Harms,the authors found that the characteristic peak of leaf is stronger than that of radix and stem at 1 656 cm-1(flavone CO),this proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem.In the two-dimensional correlation spectra,the radix and stem both have five automatic peaks(vibration of phenyl framework) in 1 350-1 700 cm-1,whereas the leaf still shows another automatic peak at 1 656 cm-1(flavone CO),and this further proves that the quantity of component of flavone in the leaf is higher than that in the radix and stem.