| 测定癌胚抗原的化学发光免疫分析新方法 |
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| 引用本文： | 庄惠生,王琼娥,陈国南,黄金陵,林培诚.测定癌胚抗原的化学发光免疫分析新方法[J].光谱学与光谱分析,2000,20(6):889-891. |
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| 作者姓名： | 庄惠生 王琼娥 陈国南 黄金陵 林培诚 |
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| 作者单位： | [1]福州大学化学系,福州 [2]福建省省立医院,福州 |
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| 基金项目： | 国家自然科学基金资助课题!(2 96750 0 3)，福建省教委科技项目!(K990 31 ) |
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| 摘 要： | 使用自制的双吖啶发光示标识别１０，１０′－二甲基－３，３′－二磺酸基－９，９′－双吖啶（简称ＤＭＤＳＢＡ）标识抗癌胚抗原（ＣＥＡ）抗体，标识率达１．１５－１．３２。研究了标识反应、双抗夹心免疫反应和化学发光反应的条件，建立了一种测定ＣＥＡ的双抗夹心免疫分析新方法，测定ＣＥＡ的线性范围为１．０－１５０．０ｎｇ．ｍＬ＾－１，最小检测浓度为０．５３ｎｇ．ｍＬ＾－１。应用于人血清中ＣＥＡ含量的测定，结果与放射免疫法相关性良好。
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| 关 键 词： | 化学发光免疫分析 癌胚抗原 肿瘤 诊断 |
A New Chemiluminescence Immunoassay Method for the Determination of Cancer Embryo Antigen |
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| H Zhuang,Q Wang,G Chen,J Huang,P Lin.A New Chemiluminescence Immunoassay Method for the Determination of Cancer Embryo Antigen[J].Spectroscopy and Spectral Analysis,2000,20(6):889-891. |
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| Authors: | H Zhuang Q Wang G Chen J Huang P Lin |
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| Institution: | Department of Chemistry, Fuzhou University, 350002 Fuzhou. |
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| Abstract: | A new chemiluminescent probe 10,10'-dimethyl-3,3'-disulfo-9,9'-biacridine DMDSBA) is used to label anti-CEA antibody, the molar incorporation is 1.15-1.32 mole of DMDSBA per mol anti-CEA; the conditions of labeling reaction, sandwich immunoassay and chemiluminescent reaction are studied. Moreover, a new double antibody sandwich chemiluminescence immunoassay is firstly established, the linear calibration ranges is 1.5-150.0 ng.mL-1 and the detection limit is 0.53 ng.mL-1. This method is used to detect cancer embryo antigen (CEA) in human serum, the results are well-matched with that of radio immunoassay. |
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