核酸-甲基青莲6B分子作用体系的研究与应用

基于核酸对甲基青莲6B的减色效应，以阳离子型染料甲基青莲6B为生色探针，研究该染料与核酸的结合反应，建立了新的核酸测定体系，研究探讨了体系的作用机理。在pH＝9.0条件下，hs-DNA，sm-DNA， ct-DNA，yeast RNA的浓度与甲基青莲6B减色效应成线性关系，响应线性范围分别为0.50～4.00 μg·mL-1，0.20～5.00 μg·mL-1，0.20～5.00 μg·mL-1，0.20～4.50 μg·mL-1，检出限(3σ/K)分别为0.082，0.037，0.038，0.041 μg·mL-1。分析核酸合成样品，回收率为93.5%～105.0%。

Studies and Application of the Interaction of Pentamethyl-p-Rosaniline Hydrochloride 6B and Nucleic Acids System

A cationic dye pentamethyl-p-rosaniline hydrochloride 6B was used as a chromic probe and its binding reaction with nucleic acids was studied. In the weak alkalescent medium, the cationic dye showed a decrease in absorption at its maximal absorption peak (584.5 nm) on binding to nucleic acids. Based on this fact, a new UV-Vis spectrophotometric method for the determination of nucleic acids has been developed. Effects of ion-intensity, surfactant and organic solvent were investigated to establish optimum reaction conditions, and then nucleic acids were detected with quantitative analysis. The calibration curves of nucleic acids such as hs-DNA, smDNA, ct-DNA and yeast-RNA were linear over the ranges 0.50-4.00 microg x mL(-1), 0.20-5.00 microg x mL(-1), 0.20-5.00 microg x mL(-1) and 0.20-4.50 microg(-1) x mL(-1), respectively. The detection limits (3sigma/K) were 0.082, 0.037, 0.038 and 0.041 microg x mL(-1). In applications to quantitative analysis of nucleic acids synthesis samples, the recoveries of DNA or RNA ranged from 93.5% to 105.0%. Furthermore, the mechanism of interaction of pentamethyl-p-rosaniline hydrochloride 6B and nucleic acids system was also discussed.