含有光敏剂的人体皮肤的激光诱导荧光光谱分析

利用血红蛋白在578nm处有吸收峰以及在光动力疗法治疗鲜红斑痣过程中病变区血液含量减少的特性,根据测得的含有光敏剂的皮肤层激光诱导荧光光谱,计算了皮肤层光敏剂的荧光强度随时间的变化关系。通过光谱处理计算了624nm处光敏剂血卟啉(HpD)的荧光峰值强度。分析和计算结果表明,皮肤层中的血液含量的减少导致新的荧光光谱峰(578nm处)的产生,新荧光峰的产生造成624nm处荧光强度的增大,因此必须排除这种影响才能得到皮肤层由光敏剂产生的荧光强度。由光谱处理结果得到治疗过程中病变区光敏剂荧光强度的变化曲线。

Laser-Induced Fluorescence Analysis of Human Skin with Photosensitizer

There is a gradually rising new fluorescence spectrum peak that appears at about 578 nm in the treatment of photodynamic therapy (PUT) of port wine stain (PWS). The new fluorescence peak spreads to 700 nm and adds additional fluorescence intensity at 624 nm, the characteristic fluorescence peak of haematoporphyrin derivative (HpD). To measure the fluorescence intensity at 624 nm of HpD, a mathematic method was employed to reduce the influence of the new peak arising at 578 nm. The time-dependent fluorescence intensity of HpD within the skin was measured. Since the hemoglobin has an absorption spectrum peak at about 578 nm, we consider that the new fluorescence peak may be caused by the decrease of blood contained in the skin with PWS during PDT treatment.