曲通X-100的荧光光谱与荧光量子产率

报道了曲通X-100(TX)水溶液的荧光光谱与荧光量子产率.实验发现,在强酸性条件下,TX没有荧光,当pH＞1时,TX有稳定的强荧光,荧光激发波长为229和275nm,发射波长为302 nm.TX水溶液可产生共振荧光,共振荧光峰位于285 nm.在0.1～90 mg·L-1浓度范围内,TX荧光强度与浓度之间存在线性关系,检测限为0.1 mg·L-1.以L-色氨酸为参比,测得在激发波长280 nm处TX水溶液的荧光量子产率为0.121.

Fluorescence Spectra and Fluorescence Quantum Yield of Triton X-100

Fluorescence spectra and fluorescence quantum yield of triton X-100 (TX) aqueous solution are reported. In strong acidic solutions, TX gives no fluorescence. When pH > 1, TX gives a strong and steady fluorescence with the maximum excitation wavelengths at 229 and 275 nm, and the maximum emission wavelength at 302 nm, respectively. TX aqueous solution can produce resonance fluorescence. The resonance fluorescence peak is located at 285 nm. A linear relationship between the fluorescence intensity and TX concentration was found in the range of 0. 1-90 mg x L(-1). The detection limit of TX is 0.1 mg x L(-1). By using L-tryptophan as a reference, the fluorescence quantum yield of TX at maximum excitation wavelength 280 nm was measured to be 0.121.