| 荧光光度法测定抗生素氧氟沙星 |
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| 引用本文： | 屠一锋,柳玲宝.荧光光度法测定抗生素氧氟沙星[J].光谱学与光谱分析,2000,20(6):880-882. |
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| 作者姓名： | 屠一锋 柳玲宝 |
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| 作者单位： | [1]苏州大学化学化工系,苏州 [2]苏州大学第一附属医院,苏州 |
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| 摘 要： | 本文报道了采用荧光分光光度法测定喹喏酮类抗生素氧氟沙星，该方法有较高的灵敏度，在氧氟沙星浓度为０．５００ｐｐｍ至２５．０ｐｐｍ间，荧光强度与浓度有良好的线性关系，线性相关系数为０．９９９９，回收率为９９．０％测定结果与紫外分光光度法测量结构无显著性差异。
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| 关 键 词： | 荧光分光光度法 氧氟沙星 抗生素 药物分析 |
The Determination of Ofloxacin by Fluorescence Spectrophotometry |
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| Y Tu,L Liu.The Determination of Ofloxacin by Fluorescence Spectrophotometry[J].Spectroscopy and Spectral Analysis,2000,20(6):880-882. |
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| Authors: | Y Tu L Liu |
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| Institution: | Department of Chemistry and Chemical Engineering, Suzhou University, 215006 Suzhou. |
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| Abstract: | This paper reports the assay of ofloxacin by fluorescence spectrophotometry. The fluorescence strength of ofloxacin is proportional to its concentration within the range from 0.500-25.0 ppm. The linear regression coefficient equals to 0.9999, and the recovery ratio is 99.0%. The results of determination for the samples of ofloxacin capsule from China and Japan are very close to that determined by the ultraviolet spectrophotometry, there isn't any obviously difference which was examined by Student's test. This method is both simplicity and accuracy, also it needs only one kind of reagent. It's believable that this method would be suitable for the use in clinical medicine and pharmaceutical industry. |
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| Keywords: | Fluroescence spectrophotometry Ofloxacin Antibiotic |
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