| 壳聚糖固定化脲酶活性的X射线微区分析 |
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| 引用本文： | 马晓莉,姚子华.壳聚糖固定化脲酶活性的X射线微区分析[J].光谱学与光谱分析,2005,25(3):456-459. |
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| 作者姓名： | 马晓莉 姚子华 |
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| 作者单位： | 河北大学化学与环境科学学院，河北省分析科学技术重点实验室，河北 保定 071002 |
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| 基金项目： | 河北省自然科学基金 (1 960 61 )资助项目 |
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| 摘 要： | 利用X射线微区分析方法，对壳聚糖固定化脲酶活性进行了定位分析。筛选出了能捕捉固定化脲酶活性部位的捕捉剂BaCl2，以尿素作为底物，在Tris-HCl缓冲溶液中，底物经固定化脲酶催化产生NH3和CO2，后者和捕捉剂反应生成BaCO3沉淀, 沉积在固定化脲酶的催化活性部位，借此确定其催化活性部位。结果表明BaCl2可用做固定化脲酶活性定位的捕捉剂。同时得到了壳聚糖固定化脲酶活性定位的最佳实验条件。
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| 关 键 词： | X射线微区分析 固定化脲酶 酶活性 壳聚糖膜 |
| 文章编号： | 1000-0593(2005)03-0456-04 |
| 收稿时间： | 2004-04-06 |
| 修稿时间： | 2004年4月6日 |
X-Ray Microanalysis of the Activity of Immobilized Urease on Chitosan Membrane |
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| MA Xiao-li,YAO Zi-hua.X-Ray Microanalysis of the Activity of Immobilized Urease on Chitosan Membrane[J].Spectroscopy and Spectral Analysis,2005,25(3):456-459. |
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| Authors: | MA Xiao-li YAO Zi-hua |
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| Institution: | College of Chemistry and Environmental Science, Hebei University, Hebei Analytical Science and Technology Key Lab, Baoding 071002, China |
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| Abstract: | The localization of the activity of immobilized urease on chitosan membrane was studied by X-ray microanalysis. BaCl_2 and urea were selected as the capture and substrate respectively. The substrate was hydrolyzed by immobilized urease to produce NH_3 and CO_2 in Tris-HCl buffer(pH 7.0), and the latter was captured by BaCl_2 to form precipitate. The precipite was deposited on the active site of immobilized urease. It is shown that the method is practicable and reliable. The optimum condition for the localization of activity of immobilized urease was studied. |
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| Keywords: | X-ray microanalysis Immobilized urease Activity Chitosan membrane |
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