| 溴化乙锭探针研究劳氏青莲与脱氧核糖核酸的相互作用 |
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| 引用本文： | 刘雪平,冯素玲,潘自红,樊静.溴化乙锭探针研究劳氏青莲与脱氧核糖核酸的相互作用[J].光谱学与光谱分析,2006,26(10):1895-1898. |
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| 作者姓名： | 刘雪平 冯素玲 潘自红 樊静 |
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| 作者单位： | 河南师范大学化学与环境科学学院，河南 新乡 453007 |
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| 摘 要： | 用光谱探针溴化乙锭对劳氏青莲与脱氧核糖核酸的作用机制进行了研究。通过对体系的荧光光谱、吸收光谱及共振光散射光谱等方面的研究，结果表明，在pH为7.4的Tris-HCl缓冲溶液中，劳氏青莲与脱氧核糖核酸之间存在着嵌插作用，其结合常数为1.0×105 L·mol-1。
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| 关 键 词： | 荧光光谱 吸收光谱 共振光散射光谱 劳氏青莲 溴化乙锭探针 DNA |
| 文章编号： | 1000-0593（2006）10-1895-04 |
| 收稿时间： | 2005-05-07 |
| 修稿时间： | 2005-10-29 |
Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe |
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| LIU Xue-ping,FENG Su-ling,PAN Zi-hong,FAN Jing.Studies on the Interaction between Thionine and Deoxyribo-Nucleic Acid by Ethidium Bromide Probe[J].Spectroscopy and Spectral Analysis,2006,26(10):1895-1898. |
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| Authors: | LIU Xue-ping FENG Su-ling PAN Zi-hong FAN Jing |
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| Institution: | College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China |
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| Abstract: | The interaction between thionine and deoxyribonucleic acid was studied with ethidium bromide probe in Tris-HCl buffer solution at pH 7.4 by fluorescence spectra, electronic absorption spectra, and resonance light scattering spectra. From the obtained results, it was found obviously that the planar structure of thionine can be intercalated into the stacked base pairs of DNA, which is the major controlling factor. The binding constant was found to be 1.0 x 10(5) L x mol(-1). |
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| Keywords: | Fluorescence spectra Absorption spectra Resonance light scattering spectra Thionine Ethidium bromide probe DNA |
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