红外光谱法在草酸钙结石研究中的应用

泌尿系结石是一种世界范围的常见病。草酸钙是泌尿系结石中最常见的组分 ,尿石中的草酸钙主要是以一水草酸钙 (COM)、二水草酸钙 (COD)的形式存在。区分草酸钙结石中的COM和COD及其比例 ,对于准确诊断结石的成因和提出正确的预防其复发的方法非常重要。红外光谱法是研究泌尿系结石的一种较理想的常用方法 ,具有快速、简便、鉴定成分准确、使用样品少、可以回收等优点。文中重点综述了傅里叶变换红外光谱 (FTIR)在泌尿系结石研究中对COM和COD的定性和定量分析方法 ,并介绍了零交叉点一次导数光谱法、FTIR与四极质谱仪、FTIR与傅里叶变换拉曼光谱仪 (FTRS)联合分析尿石的方法。

The Application of Infrared Spectrophotometry on the Study of Calcium Oxalate Calculi

Urolithiasis is a common disease throughout the world. Calcium oxalate is the most common crystalline constituent of urinary calculi. Calcium oxalate occurs mainly as two different phases: calcium oxalate monohydrate (COM) and calcium oxalate dihydrate (COD). It is very helpful for clinical diagnosis to determine the contents of COD, COM, and their ratio. Infrared (IR) spectrophotometry is an ideal analytical method and is most frequently used for the determination of the composition of urinary calculi. It has many advantages such as speediness, briefness, reliable results, little sample used and that can be reclaimed etc. In this paper the qualitative and quantitative analyses of COD and COM in the study of urinary calculi by IR spectrophotometry were summarized. Also were presented the zero-crossing-point first-derivative spectroscopy, and the combined methods of Fourier transform infrared spectroscopy (FTIR) with quadrupole mass spectrometry and with Fourier transform Raman spectroscopy (FTRS).