荧光猝灭法对头孢羟氨苄和头孢拉定荷移反应研究

研究了头孢羟氨苄和头孢拉定对荧光素的荧光猝灭反应及相互作用机理.因形成荷移络合物,头孢羟氨苄和头孢拉定对荧光素的荧光强度有很强的猝灭作用.采用紫外一可见光谱、红外光谱结合量子化学计算分析了荷移反应过程,揭示了头孢菌素类药物的成键规律和反应机理.在此基础上建立了测定头孢羟氨苄和头孢拉定含量的荧光光谱法.络合物的激发波长和发射波长分别为483,517和519 nm,头孢羟氨苄和头孢拉定浓度分别在0.3～13.5 mg·L-1和0.1～1.2 mg·L-1范围内为线性关系,相关系数分别是r=0.999 7和r=0.999 3.应用该方法测定了头孢羟氨苄和头孢拉定药物制剂含量,回收率分别在99.63%～99.91%和99.71%～100.08%之间.测定结果与文献值基本吻合.

Study of Cefadroxil and Cephradine Charge Transfer Process by Fluorescence Quenching Method

A simple and sensitive fluorescence quenching method was developed for the examination of cefadroxil and cephradine.The method is based on the reaction of fluorescein with cefadroxil or cephradine,resulting in the formation of complexes with weak fluorescence,i.e.quenching the fluorescence intensity of the system.By the use of absorption spectroscopy,infrared spectroscopy and quantum chemistry calculation the reaction process of the cephalosporins and the bonding of reaction mechanism were basically analyze...