不同波长激发光对血清荧光光谱影响的实验研究

采用日本岛津荧光光度计RF5301，研究了血清的荧光光谱与激发光波长的关系。实验结果表明：在不同波长的紫外光激励下，血清产生的荧光光谱线型及峰值波长基本相同，与激励光波长无关，但荧光峰强度随激励光波长变化而变化。血清的荧光光谱有两个较强的荧光发射区，其中第一个发射区处于300～410 nm，第二个发射区处于410～530 nm。当激发光波长小于310 nm，荧光主要集中在第一发射区，荧光峰位于330和370 nm处，并产生竞争现象。当激发光波长大于250 nm时，只出现330 nm处的荧光峰，其最佳激励光波长为300 nm；当激发光波长大于320 nm，第一发射区的荧光变弱，在第二发射区的荧光变强，荧光峰位于452 nm。此研究为血液的光谱特性研究提供了实验依据，对光诱导荧光光谱诊断技术中激发光波长的选择具有一定的参考价值。

Experimental Investigation of Fluorescence Spectra of Serum Excitated with Different Wavelength Light

In the present paper the fluorescence spectra of the blood serum excited with different wavelength were measured with the fluorescence photometer RF5301 (SHIMADZU) made in Japan. The relationship between the fluorescence spectra of the serum and the wavelength of the excitation light was studied during the experiment. The experimental results show that the linetype and peak wavelength of the fluorescence spectra of serum excited by ultraviolet radiation with different wavelength are almost the same, and they do not depend on the excitation wavelength. But the fluorescence peak value changes with the excitation wavelength. There are two high intensity emission intervals in the fluorescence spectra. One of these is from about 300 nm to 410 nm, and the other is below 310 nm. The fluorescence spectra are mostly centralized in the first interval, and the wavelengths of fluorescence peaks were found around two locations: one is near 330 nm and the other is near 370 nm. At this time the strife phenomena occur. When the excitation wavelength is about 250 nm or higher, the fluorescence peak only occurs at 330 nm, and the optimal excitation wavelength is 300 nm. While the wavelength is greater than 320 nm, the fluorescence intensity of the first interval begins to fall, while that of the other augments. And at this time the peak of wavelength of fluorescence is 452 nm. This study provides the experimental foundations for advanced study and applications of the characteristics of fluorescence spectrum of blood serum, and also offers the references to the wavelength selection of excitation light in the application of the photo-induced fluorescence spectra diagnostic technology.