达旦黄与牛血清白蛋白结合的热力学研究

采用荧光光谱法和紫外-可见吸收光谱法研究了达旦黄(TY)与牛血清白蛋白(BSA)结合反应的特征。实验发现：TY对BSA有较强的荧光猝灭作用，根据荧光猝灭数据，由Stern-Volmer方程和Line weaver-Burk双倒数方程得到了反应的结合常数和热力学参数，并由此推出了结合反应的主要作用力类型。在此基础上依据Frster非辐射能量转移机理，探讨了TY与BSA相互结合时，给体-受体间的距离和能量转移效率。从而证实了该结合作用为单一静态猝灭过程，并阐明了其猝灭机制是通过能量转移产生的。

Studies on the Thermodynamics and Mechanism of Combination Reaction of Titan Yellow with Bovine Serum Albumin

The characteristics of the binding reaction of titan yellow(TY) with bovine serum albumin(BSA) were studies by fluorescence and ultraviolet-visible absorption spectra. It was shown that TY has a powerful ability to quench the fluorescence intensity of BSA. The fluorescence quenching data were analyzed according to Stern-Volmer equation and Line weaver-Burk equation and the binding constant, and thermodynamic parameters were obtained. According to the thermodynamic parameters the main sorts of binding force were determined. The binding distance and energy transfer efficiency between the donor (BSA) and the acceptor (TY) were obtained by Frster’s non-radiative energy transfer mechanism. It is confirmed that the combination reaction of TY with BSA is a single static quenching process, and their interaction may be interpreted with energy transfer mechanism.