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| 人血液荧光的光谱特性及其物质来源 | |
| 引用本文： | 李步洪,张镇西,谢树森,陈荣.人血液荧光的光谱特性及其物质来源[J].光谱学与光谱分析,2006,26(7):1310-1313. |
| 作者姓名： | 李步洪 张镇西 谢树森 陈荣 |
| 作者单位： | 1. 西安交通大学生物医学信息工程教育部重点实验室,生命科学与技术学院,生物医学工程研究所,陕西,西安,710049;福建师范大学,医学光电信息科学与技术教育部重点实验室,激光与光电子技术研究所,福建,福州,350007 2. 西安交通大学生物医学信息工程教育部重点实验室,生命科学与技术学院,生物医学工程研究所,陕西,西安,710049 3. 福建师范大学,医学光电信息科学与技术教育部重点实验室,激光与光电子技术研究所,福建,福州,350007 |
| 基金项目： | 中国博士后科学基金 , 福建省自然科学基金 |
| 摘 要： | 取健康人的静脉血,首次实验测量了人血液的三维荧光光谱,并通过分析荧光激发-发射矩阵研究了血液自体荧光的物质来源.结果表明:人血液的荧光激发-发射对主要有260-630,280-340,340-460,450-520 nm,它们所对应的内源性荧光物质分别来源于血液中的内源性卟啉,色氨酸,还原烟碱腺嘌呤二核苷酸(磷酸盐)和黄素腺嘌呤二核苷酸.这些物质的荧光激发效率随着激发光波长的变化而表现出显著差异. |
| 关 键 词： | 人血液 激光诱导荧光 吸收光谱 三维荧光光谱 激发-发射矩阵 内源性荧光物质 |
| 文章编号： | 1000-059312006）07-1310-04 |
| 收稿时间： | 2005-04-02 |
| 修稿时间： | 2005-08-17 |
Fluorescence Spectral Characteristics of Human Blood and Its Endogenous Fluorophores |
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| LI Bu-hong,ZHANG Zhen-xi,XIE Shu-sen,CHEN Rong.Fluorescence Spectral Characteristics of Human Blood and Its Endogenous Fluorophores[J].Spectroscopy and Spectral Analysis,2006,26(7):1310-1313. | |
| Authors: | LI Bu-hong ZHANG Zhen-xi XIE Shu-sen CHEN Rong |
| Institution: | Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Biomedical Engineering, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. |
| Abstract: | The three-dimensional fluorescence spectra and excitation-emission matrix of human blood were measured,and an(attempt) was made to exploit the endogenous fluorophores of major peaks in the UV and visible.The result indicates that the(absorption) peaks of human blood appear at 274,345,415,541 and 576 nm. Based on the analysis of fluorescence excitation-emission matrix,the major emission peaks of human blood occur at excitation-emission wavelength pairs of 260-630,280-340,340-460 and 450-520 nm,which are attributed to endogenous porphyrins,tryptophan,reduced nicotinamide adenine dinucleotide(NADH) or reduced nicotinamide adenine dinucleotide phosphate(NADPH),and flavin adenine dinucleotide(FAD),respectively.These results can be used to analyze and explain the effect of blood on the distortion of fluorescence signal of human tissues for optical diagnosis. |
| Keywords: | Human blood Laser-induced fluorescence Absorption spectroscopy Three-dimensional fluorescence spectroscopy Excitation-emission matrix Endogenous fluorophores |
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