氢化物发生-原子荧光光谱法测定植物样品中的硒

建立了氢化物发生-原子荧光光谱法测定植物样品中硒的分析方法。研究了试剂及预还原方式对硒原子荧光强度的影响,探讨了共存离子对硒测定的影响和消除方法。在最佳消解条件和测定条件下,硒的线性回归方程为I=139.98c+27.71,线性范围为0～10ng.mL-1,相关系数为1.000 0,检出限为1.45ng.g-1。测定标准物质中硒的回收率为98.9%～101%,其平均值为100%。对灌木枝叶样品中的硒进行分析,其相对标准偏差为0.73%(n=9)。以国家标准物质(GSV-1)为监控样品,测定值与标准值吻合。该方法具有成本低、操作简单和高效分析的优点,并成功用于植物样品中硒的测定。

Determination of selenium in plant samples by hydride generation atomic fluorescence spectrometry

A method was developed by hydride generation atomic fluorescence spectrometry (HG-AFS)for the determination of selenium in plant samples. Effects of reagent and pre-reduction method on the fluorescence intensity of selenium were studied. The influence of coexisting foreign ions on the determination of selenium was also investigated. Under the optimized digestive and experimental conditions, the linear regression equation was I = 139.98c + 27.71 for Se. The linear range, the correlation coefficient,and the detection limit of Se was 0-10 ng x mL(-1), 1.0000, and 1.45 ng x g(-1) respectively. The recovery of Se (98.9%-101%, mean=100%) was determined through the use of standard reference material. The relative standard deviation for nine replicate analyses was 0.73% for Se content in shrub leaves. This method was verified by analyzing the national reference material (GSV-1)and the found value was in good agreement with the certified value. The proposed method that was successfully used for the determination of Se in plant samples has the advantages of simple operation, low cost, and high efficiency.