| 氯酚红-蛋白体系的共振光散射法定量测定蛋白质 |
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| 引用本文： | 刘保生,张红医,张红蕾,赵勇.氯酚红-蛋白体系的共振光散射法定量测定蛋白质[J].光谱学与光谱分析,2003,23(2):229-231. |
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| 作者姓名： | 刘保生 张红医 张红蕾 赵勇 |
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| 作者单位： | 河北大学化学与环境科学学院,河北,保定,071002 |
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| 摘 要： | 研究了酸性染料氯酚红与蛋白质的结合反应。在pH为4.00的邻苯二甲酸氢钾缓冲介质中,氯酚红与蛋白质通过分子间作用力形成复合物。使最大波长约为340nm的共振光散射光谱得到加强。基于这一现象可以测定低至0.020mg·L~(-1)的血清白蛋白,工作曲线在0～0.75mg·L~(-1)范围内呈线性关系。此方法的稳定性好,灵敏度高,用于人血清试样中总蛋白的测定,与常用的双缩脲法基本一致,且灵敏度高。
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| 关 键 词： | 共振光散射 氯酚红 人血清白蛋白 牛血清白蛋白 |
| 文章编号： | 1000-0593(2003)02-0229-03 |
| 修稿时间： | 2002年10月6日 |
The High-Sensitivity Determination of Protein Concentration by the Enhancement of Resonance Light Scattering of Chlorophenol Red |
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| LIU Baosheng,ZHANG Hong-yi,ZHANG Hong-lei and ZHAO Yong
College of Chemistry and Environment Science,Hebei University,Baoding ,China.The High-Sensitivity Determination of Protein Concentration by the Enhancement of Resonance Light Scattering of Chlorophenol Red[J].Spectroscopy and Spectral Analysis,2003,23(2):229-231. |
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| Authors: | LIU Baosheng ZHANG Hong-yi ZHANG Hong-lei and ZHAO Yong
College of Chemistry and Environment Science Hebei University Baoding China |
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| Institution: | College of Chemistry and Environment Science, Hebei University, Baoding 071002, China. |
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| Abstract: | A new resonance light scattering (RLS) assay is presented in this paper. At the optimum pH = 4.0, the weak RLS of chlorophenol red can be greatly enhanced by the addition of proteins due to the interaction between protein and chlorophenol red. A new quantitative determination method for proteins has been developed. The linear range is 0-1.0 mg.L-1 for BSA, and 0-0.75 mg.L-1 for HSA with detection limit of 20.3 micrograms.L-1 for BSA, and 20.4 micrograms.L-1 for HSA, respectively. Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good stability and few inferring substances. The determination results of the proteins in human serum samples by this method are very close to those obtained using biuret spectrophotometric method, with relative stand deviation of 1.17%-2.42%. |
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| Keywords: | Protein Resonance light scattering Chlorophenol red Human serum albumin Bovine serum albumin |
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