中药白芨水浸液的荧光光谱研究

报道了白芨沸水浸取液的三维荧光光谱和紫外吸收光谱.在三维荧光光谱图中出现3个荧光峰,分别位于λex/λem=227/299 nm,271/299 nm和258/389 nm.其中前2个荧光峰具有相同的发射波长,是由同一种荧光组分产生的,后1个荧光峰足由另外一种荧光组分产生的.三维荧光光谱具有特征性,可以用于白芨的定性鉴别.两种荧光组分的发射波长相距90 nm,可以不经分离而分别测量.在稀溶液条件下,两种组分的荧光强度与浓度之间都存在良好的线性关系,据此可以对两种组分进行定量分析.在pH 1.5～12.5范围内改变溶液的pH值时,两种组分的荧光强度有明显改变,表明两种组分的分子结构中存在可以离解的质子.

Fluorescence Spectra of Bletilla Striata Aqueous Extraction

Three-dimensional fluorescence spectrum and ultraviolet absorption spectrum of Bletilla striata extraction drawn by boiling water were reported. Three fluorescence peaks, located at lambda(ex)/lambda(em) = 227/299 nm, 271/299 nm and 258/ 389 nm respectively, were observed in the three-dimensional fluorescence spectrum. Among them, the two former peaks having the same emission wavelengths were produced by one fluorescent component, while the latter peak was produced by another. Three-dimensional fluorescence spectrum is a characteristic mark of Bletilla striata, therefore it can be used in qualitative identification. The distance between emission wavelengths of the two components was 90 nm, so the fluorescence intensity of two components could be measured separately without pre-separation. For dilute solutions of Bletilla striata aqueous extraction, good linear relationships between fluorescence intensity and concentration of two components were obtained, thereby the quantitative determination of the two components may be carried out. In the range of pH 1.5 to pH 12.5, the fluorescence intensity of the two components changes with the increase in pH, indicating dissociable protons exist in the molecular structure of the two components.