| 曙红Y与牛血清白蛋白结合反应特征研究 |
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| 引用本文： | 颜承农,梅平,熊丹,刘义.曙红Y与牛血清白蛋白结合反应特征研究[J].光谱学与光谱分析,2006,26(12):2306-2310. |
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| 作者姓名： | 颜承农 梅平 熊丹 刘义 |
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| 作者单位： | 1. 长江大学化学与环境工程学院，湖北 荆州 434020
2. 武汉大学化学与分子科学学院，湖北 武汉 430072 |
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| 基金项目： | 国家自然科学基金
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湖北省自然科学基金 |
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| 摘 要： | 研究了曙红Y(EOSY)与牛血清白蛋白(BSA)作用的荧光光谱和紫外-可见吸收光谱特征，实验证明EOSY具有较强地猝灭BSA荧光强度的能力，分别用Stern-Volmer方程、Lineweaver-Burk方程和热力学方程等处理试验数据，得到了25 ℃时反应的结合常数为3.601×105 L·mol-1,反应的ΔHθ,ΔGθ和ΔSθ分别为-20.66，-31.70 kJ·mol-1和37.06 J·K-1, 为研究在人体生理条件下EOSY与BSA的结合方式、EOSY对BSA构象影响和细胞染色机理等提供了重要信息。
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| 关 键 词： | 曙红Y 牛血清白蛋白 荧光猝灭光谱 紫外吸收光谱 三维荧光光谱 热力学参数 |
| 文章编号： | 1000-0593（2006）12-2306-05 |
| 收稿时间： | 2005-10-26 |
| 修稿时间： | 2006-06-06 |
Studies on the Features of Binding Reaction of Eosin Y with Bovine Serum Albumin |
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| YAN Cheng-nong,MEI Ping,XIONG Dan,LIU Yi.Studies on the Features of Binding Reaction of Eosin Y with Bovine Serum Albumin[J].Spectroscopy and Spectral Analysis,2006,26(12):2306-2310. |
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| Authors: | YAN Cheng-nong MEI Ping XIONG Dan LIU Yi |
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| Institution: | 1. College of Chemistry and Environmental Engineering，Yangtze University, Jingzhou 434020, China2. College of Chemistry and Molecule Sciences, Wuhan University， Wuhan 430072, China |
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| Abstract: | The binding of eosin Y to bovine serum albumin (BSA) was studied by fluorescence spectroscopy and ultraviolet spectrum. It was shown that this compound has a quite strong ability to quench the fluorescence from BSA. After analyzing the fluorescence quenching data according to Sterm-Volmer equation, Lineweaver-Burk equation and thermodynamic equation at 298. 15 K, the binding constant(KLB= 3. 601 x 10(5) L x mol(-1)) and thermodynamic parameters (deltaHtheta: - 22. 66 kJ x mol(-1), deltaGtheta: -31. 30 kJ x mol(-1) and deltaStheta: 36.32 J x K(-1)were obtained, which provide important information for researching the configuration modification of BSA caused by added eosin Y, biological effects of eosin Y in a living body, and dyeing mechanism of cells. |
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| Keywords: | Eosin Y Bovine serum albumin Fluorescence spectrum Ultra-violet absorbance spectrum Three dimensional fluorescence spectrum Thermodynamic parameters |
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