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农作物种子发芽时贮藏物质动员的红外光谱研究
引用本文:刘刚,谭丽萍,董勤.农作物种子发芽时贮藏物质动员的红外光谱研究[J].光谱学与光谱分析,2008,28(1):80-83.
作者姓名:刘刚  谭丽萍  董勤
作者单位:1. 云南师范大学物理与电子信息学院,云南,昆明,650092
2. 云南大学实验中心,云南,昆明,650091
摘    要:研究种子萌发时贮藏物质动员的一般方法是化学方法,操作烦琐耗时。作者利用傅里叶变换红外光谱对豆类(大豆、蚕豆、豌豆)及禾谷类(稻谷、小麦、大麦)农作物种子发芽时贮藏物质动员情况进行了研究。结果表明,豆类种子发芽前后在1200~1000cm-1范围的光谱变化显著,表明在发芽过程中糖类物质消耗较多;禾谷类种子发芽前后蛋白质酰胺带的强度明显下降,说明发芽过程中贮藏蛋白质被利用了;另外,禾谷类种子发芽后脂类特征峰强度也下降,说明发芽过程中贮藏脂类也被动用了。红外光谱法在种子学研究方面具有快速、方便、不需对样品进行分离提取等特点。

关 键 词:农作物种子  贮藏物质  发芽  傅里叶变换红外光谱
文章编号:1000-0593(2008)01-0080-04
收稿时间:2006-09-28
修稿时间:2006-12-29

Infrared Spectroscopic Study on Storage Substance Mobilization of Crop Seeds in Germination
LIU Gang,TAN Li-ping,DONG Qin.Infrared Spectroscopic Study on Storage Substance Mobilization of Crop Seeds in Germination[J].Spectroscopy and Spectral Analysis,2008,28(1):80-83.
Authors:LIU Gang  TAN Li-ping  DONG Qin
Institution:College of Physics and Electronic Information, Yunnan Normal University, Kunming 650092, China. gliu66@163.com
Abstract:The traditional method to study the storage substance mobilization of seeds during the process of germination is chemical extraction, which is troublesome and time consuming. In the present paper, Fourier transform infrared spectroscopy was used to study the storage substance mobilization of legume and cereal seeds in germination. The spectral results show that legume seeds (soybean, pea and broad bean) exhibit similar remarkable changes in the region from 1 000 to 1 200 cm-1 in germination, which suggest that the storage carbohydrates in endosperm of legume seeds are mobilized during the process of germination. Notable changes were observed on amide bands of protein and lipid band in the spectra of germinating cereal seeds (rice, wheat and barley), indicating that storage proteins and lipids in cotyledon were utilized during the process of cereal seed germination. The results suggest that vibrational spectroscopy has the advantages of rapidress and convenience in the study of crop seeds and seed physiology.
Keywords:Crop seed  Storage substance  Germination  Fourier transform infrared spectroscopy
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