| 以铽离子为荧光探针测定尿样中痕量环丙氟哌酸 |
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| 引用本文： | 王磊,桑立红,武艳琳,程秀民,苏志德.以铽离子为荧光探针测定尿样中痕量环丙氟哌酸[J].光谱学与光谱分析,2001,21(5):691-693. |
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| 作者姓名： | 王磊 桑立红 武艳琳 程秀民 苏志德 |
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| 作者单位： | 1. 山东医科大学药学院,
2. 山东大学校医院, |
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| 摘 要： | 详细研究了环丙氟哌酸同稀土离子铽形成的配合物体系的荧光光谱特性及实验条件对荧光强度的影响。在pH为6．0的条件下，配合物荧光体系可发射铽离子强的特征荧光，其最大激发和发射波长为328和545nm。以稀土离子铽为荧光探针，测定尿样中痕量的环丙氟派酸可获得满意结果。
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| 关 键 词： | 铽 环丙氟哌酸 稀土荧光探针 尿样 痕量分析 抗微生物药物 |
| 文章编号： | 1000-0593(2001)05-0691-03 |
| 修稿时间： | 2000年3月3日 |
Determination of Ciprofloxacin in Urine Using Terbium Ion as Fluorescent Probe |
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| L Wang,L H Sang,Y L Wu,X M Cheng,Z D Su,A H Yu.Determination of Ciprofloxacin in Urine Using Terbium Ion as Fluorescent Probe[J].Spectroscopy and Spectral Analysis,2001,21(5):691-693. |
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| Authors: | L Wang L H Sang Y L Wu X M Cheng Z D Su A H Yu |
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| Institution: | Department of Pharmacy, Shandong Medical University, Jinan 250012, China. |
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| Abstract: | Factors effecting the fluorescence characteristics of the reaction CPFX-Tb were studied thoroughly. The effect of the experimental conditions on the fluorescence intensity was defined. In the pH 6.0 media, CPFX-Tb complex fluorescence system can emit intrinsic fluorescence of Tb. The excitation and emission wavelengths are 328 nm and 545 nm. The linear range is 1.0 x 10(-8)-1.0 x 10(-6) mol.L-1. The regression equation is F = 1.57 + 4.87 x 10(8) c, r = 0.9999 with the detection limit of 5.0 x 10(-9) mol.L-1. It has been satisfactory for the determination of trace ciprofloxacin in urine by using terbium ion as fluorescent probe. |
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| Keywords: | Terbium ion Ciprofloxacin Rare earth fluorescent probe |
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