乙酸对DNA空间结构微观损伤的Raman光谱特征

利用Raman光谱技术从分子水平研究了乙酸对鲱鱼精DNA空间结构微观损伤的特征。实验结果表明，鲱鱼精DNA被乙酸浸润处理后，Raman特征频率与强度均发生了不同程度的变化。证明了乙酸对DNA的损伤是整个分子，包括骨架磷酸基团、脱氧核糖及碱基。不仅是DNA的构象，而且其构型也发生了变化：氢键断裂，B型构象被修改，DNA单、双链断裂，碱基也受到了严重损伤。其碱基损伤严重程度依次为胞嘧啶、胸腺嘧啶、鸟嘌呤和腺膘呤。

Raman Spectroscopic Study of Microscopic Damage on the Space Structure of DNA with Acetic Acid

The character of microscopic damage on the space structure of the herring sperm DNA by wetting treatment of acetic acid with Raman spectroscopic technique was reported.The Raman characteristic frequency and intensity were changed in different degree after wetting treatment on DNA by acetic acid.The results showed that the entire DNA molecule was damaged by acetic acid including backbone phosphodiester groups,deoxyribose and base stacking.The conformation and forms of DNA were changed,hydrogen bond was broken,B form was modified,both single and double chains were disrupted.Base pairs were damaged.The damage degrees of the base pairs in turn were cytosine,thymine,guanine and adenine.